Molecular evolution and functional modification of plant miRNAs with CRISPR

F Deng, F Zeng, Q Shen, A Abbas, J Cheng… - Trends in plant …, 2022 - cell.com
Gene editing using clustered regularly interspaced short palindromic repeat/CRISPR-
associated proteins (CRISPR/Cas) has revolutionized biotechnology and provides genetic …

Targeting non-coding RNAs in plants with the CRISPR-Cas technology is a challenge yet worth accepting

J Basak, C Nithin - Frontiers in plant science, 2015 - frontiersin.org
Non-coding RNAs (ncRNAs) have emerged as versatile master regulator of biological
functions in recent years. MicroRNAs (miRNAs) are small endogenous ncRNAs of 18–24 …

Using CRISPR/Cas to enhance gene expression for crop trait improvement by editing miRNA targets

SS Ferreira, RS Reis - Journal of experimental botany, 2023 - academic.oup.com
Gene editing tools such as CRISPR/Cas9 are often thought of as a means to prevent gene
expression; however, a more subtle and yet powerful approach is the enhancement of gene …

CRISPR-Cas9 based genome editing reveals new insights into microRNA function and regulation in rice

J Zhou, K Deng, Y Cheng, Z Zhong, L Tian… - Frontiers in Plant …, 2017 - frontiersin.org
MicroRNAs (miRNAs) are small non-coding RNAs that play important roles in plant
development and stress responses. Loss-of-function analysis of miRNA genes has been …

Applications of CRISPR/Cas13-based RNA editing in plants

NR Kavuri, M Ramasamy, Y Qi, K Mandadi - Cells, 2022 - mdpi.com
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-
associated (Cas) system is widely used as a genome-editing tool in various organisms …

CRISPR/Cas9 in plants: at play in the genome and at work for crop improvement

B Hussain, SJ Lucas, H Budak - Briefings in functional genomics, 2018 - academic.oup.com
The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9
(CRISPR/Cas9) system uses single-guide RNAs for genome editing, making it a simple …

Derepression of specific miRNA-target genes in rice using CRISPR/Cas9

Y Lin, Y Zhu, Y Cui, R Chen, Z Chen, G Li… - Journal of …, 2021 - academic.oup.com
MicroRNAs (miRNAs) target specific mRNA molecules based on sequence complementarity
for their degradation or repression of translation, thereby regulating various developmental …

Gene editing of non-coding regulatory DNA and its application in crop improvement

YH Chen, J Lu, X Yang, LC Huang… - Journal of …, 2023 - academic.oup.com
The development of the clustered regularly interspaced short palindromic repeats/CRISPR-
associated protein (CRISPR/Cas) system has provided precise and efficient strategies to …

Disruption of miRNA sequences by TALENs and CRISPR/Cas9 induces varied lengths of miRNA production

H Bi, Q Fei, R Li, B Liu, R Xia, SN Char… - Plant Biotechnology …, 2020 - Wiley Online Library
Summary MicroRNAs (miRNAs) are 20‐24 nucleotides (nt) small RNAs functioning in
eukaryotes. The length and sequence of miRNAs are not only related to the biogenesis of …

The emerging and uncultivated potential of CRISPR technology in plant science

Y Zhang, AA Malzahn, S Sretenovic, Y Qi - Nature Plants, 2019 - nature.com
The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …