Assessing microbial diversity in Yellowstone National Park hot springs using a field deployable automated nucleic acid extraction system

JM Wood, C Urbaniak, C Parker, NK Singh… - Frontiers in Ecology …, 2024 - frontiersin.org
Microbial diversity estimation involves extracting nucleic acids from intricate sample
matrices. Preparing nucleic acid samples is time-consuming, necessitating effective cell lysis …

Detection and analysis of elusive members of a novel and diverse archaeal community within a thermal spring streamer consortium

DR Colman, R Thomas, KR Maas, CD Takacs-Vesbach - Extremophiles, 2015 - Springer
Recent metagenomic analyses of Yellowstone National Park (YNP) thermal spring
communities suggested the presence of minor archaeal populations that simultaneous PCR …

Microbial community analysis of pH 4 thermal springs in Yellowstone National Park

X Jiang, CD Takacs-Vesbach - Extremophiles, 2017 - Springer
The pH of the majority of thermal springs in Yellowstone National Park (YNP) is from 1 to 3
and 6 to 10; relatively few springs (~ 5%) have a pH range of 4–5. We used 16S rRNA gene …

Longitudinal analysis of the Five Sisters hot springs in Yellowstone National Park reveals a dynamic thermoalkaline environment

JT Peach, RC Mueller, DJ Skorupa, MM Mesle… - Scientific Reports, 2022 - nature.com
Research focused on microbial populations of thermoalkaline springs has been driven in a
large part by the lure of discovering functional enzymes with industrial applications in high …

Using custom-built primers and nanopore sequencing to evaluate CO-utilizer bacterial and archaeal populations linked to bioH2 production

İ Akaçin, Ş Ersoy, O Doluca, M Güngörmüşler - Scientific Reports, 2023 - nature.com
The microbial community composition of five distinct thermophilic hot springs was effectively
described in this work, using broad-coverage nanopore sequencing (ONT MinION …

Dissecting the dominant hot spring microbial populations based on community-wide sampling at single-cell genomic resolution

RM Bowers, S Nayfach, F Schulz… - The ISME …, 2022 - academic.oup.com
With advances in DNA sequencing and miniaturized molecular biology workflows, rapid and
affordable sequencing of single-cell genomes has become a reality. Compared to 16S rRNA …

[图书][B] Diversity of understudied archaeal and bacterial populations from Yellowstone National Park: from genes to genomes

DR Colman - 2015 - search.proquest.com
Abstract Yellowstone National Park (YNP) thermal springs have been a crucial resource for
the discovery and characterization of microbial biodiversity. The use of cultivation …

Genomic and metagenomic insights into the microbial community of a thermal spring

R Pedron, A Esposito, I Bianconi, E Pasolli, A Tett… - Microbiome, 2019 - Springer
Background Water springs provide important ecosystem services including drinking water
supply, recreation, and balneotherapy, but their microbial communities remain largely …

The impact of temperature, pH and environmental heterogeneity on prokaryotic diversity in Yellowstone National Park thermal springs

X Jiang - 2015 - digitalrepository.unm.edu
Abstract Yellowstone National Park (YNP) is one of the largest and most diverse
hydrothermal areas on Earth. Extensive culture-independent studies in YNP thermal springs …

Archaeal and bacterial communities in three alkaline hot springs in Heart Lake Geyser Basin, Yellowstone National Park

K Bowen De León, R Gerlach, BM Peyton… - Frontiers in …, 2013 - frontiersin.org
The Heart Lake Geyser Basin (HLGB) is remotely located at the base of Mount Sheridan in
southern Yellowstone National Park (YNP), Wyoming, USA and is situated along Witch …