Transgenic animals are extensively used to study in vivo gene function as well as to model
human diseases. The technology for producing transgenic animals exists for a variety of …

Modern biomedical research is gradually tightening its grip on the genetic basis of common
diseases by studying complete genomes, transcriptomes, and other complex biological …

Cell biology research encompasses everything from single cells to whole animals. Recent
discoveries concerning particular gene functions can be applied to the whole animal for …

The development of gene targeting technology, the exchange of an endogenous allele of a
target gene for a mutated copy via homologous recombination, and the application of this …

The mouse is the foremost vertebrate experimental model because its genome can be
precisely and variously engineered. Now that the mouse genome has been sequenced and …

The technique of gene targeting allows for the introduction of engineered genetic mutations
into a mouse at a determined genomic locus. The process of generating mouse models with …

Genetic modification of endogenous genes in mice has become possible by applying gene
targeting techniques to embryonic stem (ES) cells and using specific clones of cells to …

Over the past decade, new methods and procedures have been developed to generate
genetically engineered mouse models of human disease. This At a Glance article highlights …

Until recently, precise modification of the animal genome by gene targeting was restricted to
the mouse because germline competent embryonic stem cells are not available in any other …

Although in vivo models utilizing endogenous reporter genes have been exploited for many
years, the use of reporter transgenes to dissect biological issues in transgenic animals has …