Effect of heat inactivation for the detection of severe acute respiratory syndrome-corona virus-2 (SARS-CoV-2) with reverse transcription real time polymerase chain …

B Woldesemayat, G Gebremicael, K Zealiyas… - BMC Infectious …, 2022 - Springer
Abstract Background Coronavirus disease 2019 (COVID-19) has been a major public health
importance and its specimen needs to be handled safely due to concerns of potential …

Effect of heat inactivation and bulk lysis on real-time reverse transcription PCR detection of the SARS-COV-2: an experimental study

D Leta, G Gutema, GG Hagos, R Diriba, G Bulti… - BMC Research …, 2022 - Springer
Objective This study aimed to investigate the effect of heat inactivation and chemical
bulklysis on SARS-CoV-2 detection. Results About 6.2%(5/80) of samples were changed to …

Effect of heat inactivation on real-time reverse transcription PCR of the SARS-CoV-2 detection

Y Liu, Z Cao, M Chen, Y Zhong, Y Luo, G Shi, H Xiang… - medRxiv, 2020 - medrxiv.org
Background Real-time reverse transcription PCR (rRT-PCR) is commonly used to diagnose
SARS-CoV-2 infection. Heat inactivation prior to nucleic acid isolation may allow safe …

Potential false-negative nucleic acid testing results for severe acute respiratory syndrome coronavirus 2 from thermal inactivation of samples with low viral loads

Y Pan, L Long, D Zhang, T Yuan, S Cui… - Clinical …, 2020 - academic.oup.com
Abstract Background Coronavirus disease-2019 (COVID-19) has spread widely throughout
the world since the end of 2019. Nucleic acid testing (NAT) has played an important role in …

[HTML][HTML] Rapid and visual detection of 2019 novel coronavirus (SARS-CoV-2) by a reverse transcription loop-mediated isothermal amplification assay

C Yan, J Cui, L Huang, B Du, L Chen, G Xue… - Clinical Microbiology …, 2020 - Elsevier
Objective To evaluate a reverse transcription loop-mediated isothermal amplification (RT-
LAMP) assay for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV …

Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing

F Mancini, F Barbanti, M Scaturro, G Errico… - Emerging microbes & …, 2020 - Taylor & Francis
The RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples,
but it is dependent on the availability of chemical reagents. In this study, we evaluated the …

Rapid detection of novel coronavirus/Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by reverse transcription-loop-mediated isothermal …

LE Lamb, SN Bartolone, E Ward, MB Chancellor - PloS one, 2020 - journals.plos.org
Novel Corona virus/Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2 or
2019-nCoV), and the subsequent disease caused by the virus (coronavirus disease 2019 or …

Two extraction-free reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2

MY Lai, FDM Bukhari, NZ Zulkefli, I Ismail… - BMC Infectious …, 2021 - Springer
Background Current assays for detection of severe acute respiratory syndrome coronavirus
2 (SARS-CoV-2) rely on time consuming, costly and laboratory based methods for virus …

[HTML][HTML] Evaluation of extraction-free RT-PCR methods for faster and cheaper detection of SARS-CoV-2 using two commercial systems

F Morecchiato, M Coppi, I Baccani, N Maggini… - International Journal of …, 2021 - Elsevier
Objective When using high-throughput batched diagnostic platforms based on RT-PCR for
SARS-CoV-2 detection, avoidance of the conventional nucleic acid extraction step can help …

Laboratory evaluation of a SARS-CoV-2 RT-LAMP test

S Menting, A Erhart, HDFH Schallig - Tropical Medicine and Infectious …, 2023 - mdpi.com
There is a need to have more accessible molecular diagnostic tests for the diagnosis of
severe acute respiratory syndrome coronavirus 2 disease in low-and middle-income …