A fundamental challenge in biology is understanding the molecular details of protein
function. How mutations alter protein activity, regulation, and response to drugs is of critical …

Protein mutagenesis is essential for unveiling the molecular mechanisms underlying protein
function in health, disease, and evolution. In the past decade, deep mutational scanning …

Mutagenesis provides insight into proteins, but only recently have assays that couple
genotype to phenotype been used to assess the activities of as many as 1 million mutant …

Over 40% of proteins in any eukaryotic genome encode intrinsically disordered regions (IDR
s) that do not adopt defined tertiary structures. Certain IDR s perform critical functions, but …

The rapid decrease in DNA sequencing cost is revolutionizing medicine and science. In
medicine, genome sequencing has revealed millions of missense variants that change …

Analysis of protein mutants is an effective means to understand their function. Protein
display is an approach that allows large numbers of mutants of a protein to be selected …

Measuring the consequences of mutation in proteins is critical to understanding their
function. These measurements are essential in such applications as protein engineering …

The high-throughput technique of deep mutational scanning (DMS) has recently made it
possible to experimentally measure the effects of all amino-acid mutations to a protein …

Massively parallel or second-generation sequencing-based genomic studies continuously
identify new genomic alterations that may lead to novel protein sequences, which are …

Amino acids fulfil a diverse range of roles in proteins, each utilising its chemical properties in
different ways in different contexts to create required functions. For example, cysteines form …