We report the application of single-molecule-based sequencing technology for high-
throughput profiling of histone modifications in mammalian cells. By obtaining over four …

After fertilization the embryonic genome is inactive until transcription is initiated during the
maternal–zygotic transition,,. This transition coincides with the formation of pluripotent cells …

Cellular function in tissue is dependent on the local environment, requiring new methods for
spatial mapping of biomolecules and cells in the tissue context. The emergence of spatial …

Higher eukaryotes must adapt a totipotent genome to specialized cell types with stable but
limited functions. One potential mechanism for lineage restriction is changes in chromatin …

New methods for the genome-wide analysis of chromatin are providing insight into its roles
in development and their underlying mechanisms. Current studies indicate that chromatin is …

DNA methylation is essential for normal development,, and has been implicated in many
pathologies including cancer,. Our knowledge about the genome-wide distribution of DNA …

Recent advances in single-cell technologies have led to the discovery of thousands of brain
cell types; however, our understanding of the gene regulatory programs in these cell types is …

DNA methylation is a crucial element in the epigenetic regulation of mammalian embryonic
development,,,,. However, its dynamic patterns have not been analysed at the genome scale …

DNase I hypersensitive sites (DHSs) are markers of regulatory DNA and have underpinned
the discovery of all classes of cis-regulatory elements including enhancers, promoters …

Abstract The Encyclopedia of DNA Elements (ENCODE) project has established a genomic
resource for mammalian development, profiling a diverse panel of mouse tissues at 8 …