Evaluation of NGS-based approaches for SARS-CoV-2 whole genome characterisation

C Charre, C Ginevra, M Sabatier, H Regue… - Virus …, 2020 - academic.oup.com
Since the beginning of the COVID-19 outbreak, SARS-CoV-2 whole-genome sequencing
(WGS) has been performed at unprecedented rate worldwide with the use of very diverse …

A benchmarking study of SARS-CoV-2 whole-genome sequencing protocols using COVID-19 patient samples

T Liu, Z Chen, W Chen, X Chen, M Hosseini, Z Yang… - IScience, 2021 - cell.com
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an emerging new type of
coronavirus that is responsible for the COVID-19 pandemic and the unprecedented global …

Multiple approaches for massively parallel sequencing of SARS-CoV-2 genomes directly from clinical samples

M Xiao, X Liu, J Ji, M Li, J Li, L Yang, W Sun, P Ren… - Genome medicine, 2020 - Springer
Abstract Background COVID-19 (coronavirus disease 2019) has caused a major epidemic
worldwide; however, much is yet to be known about the epidemiology and evolution of the …

Whole genome sequencing of SARS-CoV-2: adapting Illumina protocols for quick and accurate outbreak investigation during a pandemic

S Pillay, J Giandhari, H Tegally, E Wilkinson… - Genes, 2020 - mdpi.com
The COVID-19 pandemic has spread very fast around the world. A few days after the first
detected case in South Africa, an infection started in a large hospital outbreak in Durban …

A comparison of whole genome sequencing of SARS-CoV-2 using amplicon-based sequencing, random hexamers, and bait capture

JA Nasir, RA Kozak, P Aftanas, AR Raphenya… - Viruses, 2020 - mdpi.com
Genome sequencing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is
increasingly important to monitor the transmission and adaptive evolution of the virus. The …

[HTML][HTML] Improvements to the ARTIC multiplex PCR method for SARS-CoV-2 genome sequencing using nanopore

JR Tyson, P James, D Stoddart, N Sparks… - BioRxiv, 2020 - ncbi.nlm.nih.gov
Genome sequencing has been widely deployed to study the evolution of SARS-CoV-2 with
more than 90,000 genome sequences uploaded to the GISAID database. We published a …

CoronaHiT: high-throughput sequencing of SARS-CoV-2 genomes

DJ Baker, A Aydin, T Le-Viet, GL Kay, S Rudder… - Genome medicine, 2021 - Springer
We present CoronaHiT, a platform and throughput flexible method for sequencing SARS-
CoV-2 genomes (≤ 96 on MinION or> 96 on Illumina NextSeq) depending on changing …

Sensitive recovery of complete SARS-CoV-2 genomes from clinical samples by use of Swift Biosciences' SARS-CoV-2 multiplex amplicon sequencing panel

A Addetia, MJ Lin, V Peddu… - Journal of clinical …, 2020 - Am Soc Microbiol
Whole-genome sequencing (WGS) of severe acute respiratory syndrome coronavi-rus 2
(SARS-CoV-2) has led to a better understanding of the virus's origin, transmission, and …

Analytical validity of nanopore sequencing for rapid SARS-CoV-2 genome analysis

RA Bull, TN Adikari, JM Ferguson, JM Hammond… - Nature …, 2020 - nature.com
Viral whole-genome sequencing (WGS) provides critical insight into the transmission and
evolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Long-read …

Rapid and inexpensive whole-genome sequencing of SARS-CoV-2 using 1200 bp tiled amplicons and Oxford Nanopore Rapid Barcoding

NE Freed, M Vlková, MB Faisal… - Biology Methods and …, 2020 - academic.oup.com
Rapid and cost-efficient whole-genome sequencing of severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2), the virus that causes coronavirus disease 2019, is critical for …