Although the highly dynamic and mosaic organization of the plasma membrane is well-
recognized, depicting a resolved, global view of this organization remains challenging. We …

Single-particle tracking (SPT) is often the rate-limiting step in live-cell imaging studies of
subcellular dynamics. Here we present a tracking algorithm that addresses the principal …

Optical microscopy has for centuries been a key tool to study living cells with minimum
invasiveness. The advent of single molecule techniques over the past two decades has …

Single particle tracking (SPT) in biological systems is a quickly growing field. Many new
technologies are being developed providing new tracking capabilities, which also lead to …

In recent years, the development of fast and highly sensitive microscopy has changed the
way of thinking of cell biologists: it became more and more important to study the structural …

To the Editor: Many contemporary models of the plasma membrane are based on single-
particle tracking (SPT) by light microscopy on live cells. Whereas the analysis of single …

To the Editor: Single-particle tracking (SPT) grants unprecedented insight into cellular
function at the molecular scale1. Throughout the cell, the movement of single molecules is …

We combined photoactivated localization microscopy (PALM) with live-cell single-particle
tracking to create a new method termed sptPALM. We created spatially resolved maps of …

Single-particle tracking (SPT) directly measures the dynamics of proteins in living cells and
is a powerful tool to dissect molecular mechanisms of cellular regulation. Interpretation of …

Molecular trafficking within cells, tissues and engineered three-dimensional multicellular
models is critical to the understanding of the development and treatment of various diseases …