Production of hydroxytyrosol rich extract from Olea europaea leaf with enhanced biological activity using immobilized enzyme reactors

AV Chatzikonstantinou, Α Giannakopoulou… - … Science and Pollution …, 2022 - Springer
As olive leaves constitute the main by-product of the olive oil industry with important
environmental and economic impact, there is an increasing demand for its valorization. In …

Production of highly purified hydroxytyrosol from Olea europaea leaf extract biotransformed by hyperthermophilic β-glycosidase

R Briante, M Patumi, F Febbraio, R Nucci - Journal of Biotechnology, 2004 - Elsevier
A large amount of highly purified hydroxytyrosol (91–94% in weight) is obtained in short time
by a simple biotransformation of Olea europaea leaf extract by a partially purified …

Continuous bioconversion of oleuropein from olive leaf extract to produce the bioactive product hydroxytyrosol using carrier-immobilized enzyme

M Liu, Q Yong, Z Lian, C Huang, S Yu - Applied biochemistry and …, 2020 - Springer
Feasibility and stability were evaluated of a continuous multi-batch process for converting
oleuropein (OLE) from olive leaf extract to the bioactive product hydroxytyrosol (HT). Carrier …

Efficient 2-step enzymatic cascade for the bioconversion of oleuropein into hydroxytyrosol

G Catinella, S Donzella, G Borgonovo, S Dallavalle… - Antioxidants, 2022 - mdpi.com
Among the plant bioactive components, oleuropein (OLE) is the most abundant phenolic
compound in all parts of olive trees (Olea europaea L.), particularly concentrated in olive …

Development of a novel bi-enzymatic nanobiocatalyst for the efficient bioconversion of oleuropein to hydroxytyrosol

A Giannakopoulou, AV Chatzikonstantinou… - Catalysts, 2021 - mdpi.com
Lipase A from Candida antarctica (CalA) and β-glucosidase from Thermotoga maritima (bgl)
were covalently co-immobilized onto the surface of chitosan-coated magnetic nanoparticles …

Bioactive derivatives from oleuropein by a biotransformation on Olea europaea leaf extracts

R Briante, F La Cara, F Febbraio, M Patumi… - Journal of …, 2002 - Elsevier
A very simple method is proposed to produce, using non-homogeneous hyperthermophilic β-
glycosidase immobilised on chitosan, 3, 4-dihydroxy-phenylethanol (hydroxytyrosol), a …

Bioactivity Improvement of Olea europaea Leaf Extract Biotransformed by Wickerhamomyces anomalus Enzymes

R Palmeri, C Restuccia, JI Monteleone… - Plant Foods for Human …, 2017 - Springer
Olive leaves represent a quantitatively significant by-product of agroindustry. They are rich in
phenols, mainly oleuropein, which can be hydrolyzed into several bioactive compounds …

Microbial application to improve olive mill wastewater phenolic extracts

FV Romeo, G Granuzzo, P Foti, G Ballistreri, C Caggia… - Molecules, 2021 - mdpi.com
Olive mill wastewater (OMW) contains valuable and interesting bioactive compounds,
among which is hydroxytyrosol, which is characterized by a remarkable antioxidant activity …

Enzymatic tailoring of oleuropein from Olea europaea leaves and product identification by HRMS/MS spectrometry

E Nikolaivits, A Termentzi, AL Skaltsounis… - Journal of …, 2017 - Elsevier
Oleuropein, a bioactive compound found in all parts of olive tree, especially in leaves and
branches, presents numerous health promoting properties that increase research and …

Biorefinery of olive leaves to produce dry oleuropein aglycone: Use of homemade ceramic capillary biocatalytic membranes in a multiphase system

G Ranieri, R Mazzei, T Poerio, F Bazzarelli… - Chemical Engineering …, 2018 - Elsevier
Oleuropein aglycone is an important antioxidant compound produced during oleuropein
hydrolysis, not yet commercially available. Its production from renewable material by green …