[HTML][HTML] Production and certification of NIST standard reference material 2372 human DNA quantitation standard

MC Kline, DL Duewer, JC Travis, MV Smith… - Analytical and …, 2009 - Springer
Modern highly multiplexed short tandem repeat (STR) assays used by the forensic human-
identity community require tight control of the initial amount of sample DNA amplified in the …

[HTML][HTML] Human DNA quantification and sample quality assessment: Developmental validation of the PowerQuantĘr) system

MM Ewing, JM Thompson, RS McLaren… - Forensic Science …, 2016 - Elsevier
Quantification of the total amount of human DNA isolated from a forensic evidence item is
crucial for DNA normalization prior to short tandem repeat (STR) DNA analysis and a federal …

[PDF][PDF] Results from the NIST 2004 DNA quantitation study

MC Kline, JW Redman, JM Butler… - Journal of forensic …, 2005 - archive.gfjc.fiu.edu
For optimal DNA short tandem repeat (STR) typing results, the DNA concentration ([DNA]) of
the sample must be accurately determined prior to the polymerase chain reaction (PCR) …

Validation of reduced‐scale reactions for the Quantifiler™ human DNA kit

CG Westring, R Kristinsson, DM Gilbert… - Journal of forensic …, 2007 - Wiley Online Library
Accurate quantification of DNA samples is an important step in obtaining accurate and
reproducible short tandem repeat (STR) profiles. Quantitative real‐time‐PCR has improved …

[HTML][HTML] Evaluating droplet digital PCR for the quantification of human genomic DNA: converting copies per nanoliter to nanograms nuclear DNA per microliter

DL Duewer, MC Kline, EL Romsos, B Toman - Analytical and bioanalytical …, 2018 - Springer
The highly multiplexed polymerase chain reaction (PCR) assays used for forensic human
identification perform best when used with an accurately determined quantity of input DNA …

Development and validation of the Investigator® Quantiplex Pro Kit for qPCR-based examination of the quantity and quality of human DNA in forensic samples

M Vraneš, M Scherer, K Elliott - Forensic Science International: Genetics …, 2017 - Elsevier
We developed and validated the Investigator Quantiplex Pro Kit which quantifies human and
male DNA in a sample using quantitative real-time PCR. An internal PCR control (IPC) is …

Developmental validation of a real-time quantitative PCR assay for automated quantification of human DNA

ML Richard, RH Frappier, JC Newman - Journal of forensic sciences, 2003 - astm.org
Our laboratory has developed an automated real-time quantitative PCR assay for detecting
human DNA. The assay utilizes an inhouse, custom-designed TaqMan®-MGB sequence …

Certification of standard reference Material® 2372a human DNA quantitation standard

EL Romsos, MC Kline, N Farkas, DL Duewer, B Toman - 2018 - nist.gov
Abstract Standard Reference Material (SRM) 2372a is intended for use in the value
assignment of human genomic deoxyribonucleic acid (DNA) forensic quantitation materials …

Development and validation of InnoQuant® HY, a system for quantitation and quality assessment of total human and male DNA using high copy targets

A Loftus, G Murphy, H Brown, A Montgomery… - Forensic Science …, 2017 - Elsevier
The development and validation of InnoQuant® HY, a real-time PCR system containing four
DNA targets—two RE autosomal targets of different sizes, male specific targets, and an …

An analysis of single and multi-copy methods for DNA quantitation by real-time polymerase chain reaction

HE LaSalle, G Duncan, B McCord - Forensic Science International …, 2011 - Elsevier
The goal of this paper was to examine and compare two different commercially available
approaches to the determination of the relative quantities of autosomal and Y chromosomal …