Circular intensity differential scattering for label-free chromatin characterization: A review for optical microscopy

A Le Gratiet, R Marongiu, A Diaspro - Polymers, 2020 - mdpi.com
Circular Intensity Differential Scattering (CIDS) provides a differential measurement of the
circular right and left polarized light and has been proven to be a gold standard label-free …

Circular intensity differential scattering (CIDS) scanning microscopy to image chromatin-DNA nuclear organization

A Le Gratiet, L Pesce, M Oneto, R Marongiu… - OSA …, 2018 - opg.optica.org
Circular dichroism imaging has proved a powerful and simple method for extracting
information on chiral molecules without specific fluorescent labels. Numerous mathematical …

Combined approach using circular intensity differential scattering microscopy under phasor map data analysis

A Mohebi, A Le Gratiet, R Marongiu, F Callegari… - Applied Optics, 2021 - opg.optica.org
Circular intensity differential scattering (CIDS) is based on the analysis of circular polarized
light scattering and has been proven to be an interesting label-free microscopy technique …

ExCIDS: a combined approach coupling Expansion Microscopy (ExM) and Circular Intensity Differential Scattering (CIDS) for chromatin-DNA imaging

R Marongiu, A Le Gratiet, L Pesce, P Bianchini… - OSA …, 2020 - opg.optica.org
Expansion microscopy (ExM) is a novel preparation method enhancing the optical resolution
by expanding uniformly the relative distance between fluorescence molecules on a sample …

Differential scattering of circularly polarized light by chromatin modeled as a helical array of dielectric ellipsoids within the born approximation

AS Belmont, S Zietz, C Nicolini - … : Original Research on …, 1985 - Wiley Online Library
Theoretical predictions within the Born approximation of the expected differential light
scattering of circularly polarized light (CIDS) were made for the 300‐Å chromatin fiber …

[HTML][HTML] On the structural organization of macromolecules using chiral sensitive differential scattering of circularly polarized light

MW Ashraf, A Diaspro - Optics Communications, 2022 - Elsevier
Differential scattering of circularly polarized light is demonstrated to characterize the
macromolecular structures consisting of hierarchical chirality. We modeled the B-DNA …

Electronic circular dichroism for chiral analysis

J Gawronski, P Skowronek - Chiral Analysis, 2006 - Elsevier
Publisher Summary This chapter discusses circular dichroism (CD) for chiral analysis. CD is
a spectroscopic technique based on differential absorption of left-and right-handed circularly …

Circular dichroism imaging: mapping the local supramolecular order in thin films of chiral functional polymers

F Zinna, C Resta, M Gorecki, G Pescitelli… - …, 2017 - ACS Publications
We introduce the concept of circular dichroism (CD) imaging CD i, which is made possible
by the use of the highly collimated light beam of synchrotron radiation (SR) only available at …

Structural analysis of biomacromolecules using circular dichroism spectroscopy

X Zhao, Y Wang, D Zhao - … Methods to Study Biomolecular Structure and …, 2023 - Elsevier
The chirality of biomacromolecules renders circular dichroism (CD) detection a method to
measure their multiscale structures. This chapter reviews the applications of CD …

Computational modeling of chromatin fiber to characterize its organization using angle-resolved scattering of circularly polarized light

MW Ashraf, A Le Gratiet, A Diaspro - Polymers, 2021 - mdpi.com
Understanding the structural organization of chromatin is essential to comprehend the gene
functions. The chromatin organization changes in the cell cycle, and it conforms to various …