RNA sequencing: advances, challenges and opportunities

F Ozsolak, PM Milos - Nature reviews genetics, 2011 - nature.com
In the few years since its initial application, massively parallel cDNA sequencing, or RNA-
seq, has allowed many advances in the characterization and quantification of …

RNA sequencing and analysis

KR Kukurba, SB Montgomery - Cold Spring Harbor Protocols, 2015 - cshprotocols.cshlp.org
RNA sequencing (RNA-Seq) uses the capabilities of high-throughput sequencing methods
to provide insight into the transcriptome of a cell. Compared to previous Sanger sequencing …

Defining transcribed regions using RNA-seq

BT Wilhelm, S Marguerat, I Goodhead, J Bähler - Nature protocols, 2010 - nature.com
Next-generation sequencing technologies are revolutionizing genomics research. It is now
possible to generate gigabase pairs of DNA sequence within a week without time …

RNA-Seq: a revolutionary tool for transcriptomics

Z Wang, M Gerstein, M Snyder - Nature reviews genetics, 2009 - nature.com
RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-
sequencing technologies. Studies using this method have already altered our view of the …

RNA sequencing: the teenage years

R Stark, M Grzelak, J Hadfield - Nature Reviews Genetics, 2019 - nature.com
Over the past decade, RNA sequencing (RNA-seq) has become an indispensable tool for
transcriptome-wide analysis of differential gene expression and differential splicing of …

Informatics for RNA sequencing: a web resource for analysis on the cloud

M Griffith, JR Walker, NC Spies… - PLoS computational …, 2015 - journals.plos.org
Massively parallel RNA sequencing (RNA-seq) has rapidly become the assay of choice for
interrogating RNA transcript abundance and diversity. This article provides a detailed …

RNA‐Seq methods for transcriptome analysis

R Hrdlickova, M Toloue, B Tian - Wiley Interdisciplinary …, 2017 - Wiley Online Library
Deep sequencing has been revolutionizing biology and medicine in recent years, providing
single base‐level precision for our understanding of nucleic acid sequences in high …

RNA‐seq data: challenges in and recommendations for experimental design and analysis

AG Williams, S Thomas, SK Wyman… - Current protocols in …, 2014 - Wiley Online Library
RNA‐seq is widely used to determine differential expression of genes or transcripts as well
as identify novel transcripts, identify allele‐specific expression, and precisely measure …

Advanced applications of RNA sequencing and challenges

Y Han, S Gao, K Muegge, W Zhang… - … and biology insights, 2015 - journals.sagepub.com
Next-generation sequencing technologies have revolutionarily advanced sequence-based
research with the advantages of high-throughput, high-sensitivity, and high-speed. RNA-seq …

RNA-seq: from technology to biology

S Marguerat, J Bähler - Cellular and molecular life sciences, 2010 - Springer
Next-generation sequencing technologies are now being exploited not only to analyse static
genomes, but also dynamic transcriptomes in an approach termed RNA-seq. Although these …