Introduction: Facemasks carrying a gelatine sampling matrix have been used to sample
exhalations from TB patients. The aim here was to further develop this approach by …

Methods: amplified using NEB Phusion enzyme and a broad range of eubacterial primers.
Chain termination DNA sequencing was performed using a three primer protocol. A total of …

The lack of methodological standardization diminishes the validity of results obtained and
the conclusions drawn when studying the lung microbiota. We report the validation of a …

Breath contains numerous classes of compounds and biomolecules that could potentially be
used as biomarkers for infectious disease as well as a range of other respiratory conditions …

Background The management of ventilator-associated and hospital-acquired pneumonia
requires rapid and accurate quantitative detection of the infecting pathogen (s). To do it, we …

RATIONALE Lower Respiratory Tract Infections (LRTIs) are the fifth leading cause of death,
accounting for an estimated 2.74 million fatalities in 2015. Traditional microbiological …

Background Bioaerosols from pulmonary tuberculosis (PTB) patients are a quantitative
predictor of transmission. Current methods involve sophisticated instruments and time …

The management of ventilator-associated and hospital-acquired pneumonia requires rapid
and accurate quantitative detection of the infecting pathogen (s). To achieve this, we …

Novel sampling matrices were manufactured using 3D printing for the detection of
respiratory pathogens in expired air. A specific configuration of the matrices was designed …

Rationale: Metagenomic sequencing of respiratory microbial communities has shown
promise for developing novel pneumonia diagnostics needed to overcome the well …