This study provides an assessment of the Fluidigm C1 platform for RNA sequencing of
single mouse pancreatic islet cells. The system combines microfluidic technology and …

Pancreatic islets consist of multiple cell types that produce hormones required for glucose
homeostasis, and islet dysfunction is a major factor in type 1 and type 2 diabetes. Numerous …

Understanding the transcriptional heterogeneity that occurs on the level of a single cell is
critical to understanding the gene‐regulatory mechanisms underlying development and …

Background Single-cell RNA sequencing (scRNA-seq) provides valuable insights into
human islet cell types and their corresponding stable gene expression profiles. However …

Background Technological advances have enabled transcriptome characterization of cell
types at the single-cell level providing new biological insights. New methods that enable …

Background Human pancreatic islets are a central focus of research in metabolic studies.
Transcriptomics is frequently used to interrogate alterations in cultured human islet cells …

Pancreatic islets secrete insulin from β cells and glucagon from α cells, and dysregulated
secretion of these hormones is a central component of diabetes. Thus, an improved …

In the past 3 years, we have seen a flurry of publications on single-cell RNA sequencing
(RNA-seq) analyses of pancreatic islets from mouse and human. This technology holds the …

Pancreatic islet cells are critical for maintaining normal blood glucose levels, and their
malfunction underlies diabetes development and progression. We used single-cell RNA …

To understand organ function, it is important to have an inventory of its cell types and of their
corresponding marker genes. This is a particularly challenging task for human tissues like …