Background: Reagent proteins such as DNA ligases play a central role in the global
reagents market. DNA ligases are commonly used and are vital in academic and science …

The DNA ligase from bacteriophage T4 is one of the most widely used enzymes in molecular
biology. It has evolved to seal single-stranded nicks in double-stranded DNA, but not to join …

DNA ligases have numerous applications in molecular biology and biotechnology. However,
many of these applications require the ligation of blunt-ended DNA termini, which is an …

A new rapid assay method for DNA ligases has been developed, which allows direct
quantification of enzyme activity without using the traditional polyacrylamide gel …

DNA ligases are fundamental enzymes in molecular biology and biotechnology where they
perform essential reactions, eg to create recombinant DNA and for adaptor attachment in …

Ligases are gaining utility in molecular biology applications, such as nucleotide sequence
detection, single nucleotide polymorphism (SNP) detection, protein detection and “next …

DNA ligases play an essential role in DNA replication, repair, and recombination and are
classified as ATP-dependent or NAD-dependent based on their adenylation cofactor …

DNA ligases are indispensable in all living cells and ubiquitous in all organs. DNA ligases
are broadly utilized in molecular biology research fields, such as genetic engineering and …

DNA ligases join breaks in the phosphodiester backbone of DNA by catalysing the formation
of bonds between opposing 5′ P and 3′ OH ends in an adenylation-dependent manner …

The DNA ligase enzyme family catalyzes the formation of a phosphodiester bond between
juxtaposed 5′‐phosphate and 3′‐hydroxyl termini in duplex DNA. This activity can seal …