Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles

U Erdbrügger, CJ Blijdorp, IV Bijnsdorp… - Journal of …, 2021 - Wiley Online Library
Urine is commonly used for clinical diagnosis and biomedical research. The discovery of
extracellular vesicles (EV) in urine opened a new fast‐growing scientific field. In the last …

Urinary extracellular vesicles and the kidney: biomarkers and beyond

M Salih, R Zietse, EJ Hoorn - American Journal of …, 2014 - journals.physiology.org
Extracellular vesicles have been isolated in various body fluids, including urine. The cargo
of urinary extracellular vesicles (uEVs) is composed of proteins and nucleic acids reflecting …

Urinary extracellular vesicles: Origin, role as intercellular messengers and biomarkers; efficient sorting and potential treatment options

P Svenningsen, R Sabaratnam, BL Jensen - Acta Physiologica, 2020 - Wiley Online Library
Urinary extracellular vesicles (uEVs) are a heterogenous group of vesicles consisting mainly
of microvesicles and exosomes that originate predominantly (99.96%) from kidney, the …

[HTML][HTML] Rigorous characterization of urinary extracellular vesicles (uEVs) in the low centrifugation pellet-a neglected source for uEVs

L Musante, SV Bontha, S La Salvia… - Scientific Reports, 2020 - nature.com
Urinary extracellular vesicles (uEVs) provide bio-markers for kidney and urogenital
diseases. Centrifugation is the most common method used to enrich uEVs. However, a …

Urinary extracellular vesicles: Assessment of pre‐analytical variables and development of a quality control with focus on transcriptomic biomarker research

K Barreiro, OP Dwivedi, S Valkonen… - Journal of …, 2021 - Wiley Online Library
Urinary extracellular vesicles (uEV) are a topical source of non‐invasive biomarkers for
health and diseases of the urogenital system. However, several challenges have become …

Isolation and characterization of urinary extracellular vesicles: implications for biomarker discovery

ML Merchant, IM Rood, JKJ Deegens… - Nature Reviews …, 2017 - nature.com
Urine is a valuable diagnostic medium and, with the discovery of urinary extracellular
vesicles, is viewed as a dynamic bioactive fluid. Extracellular vesicles are lipid-enclosed …

Comparing approaches to normalize, quantify, and characterize urinary extracellular vesicles

CJ Blijdorp, OAZ Tutakhel, TA Hartjes… - Journal of the …, 2021 - journals.lww.com
Background Urinary extracellular vesicles (uEVs) are a promising source for biomarker
discovery, but optimal approaches for normalization, quantification, and characterization in …

Different EV enrichment methods suitable for clinical settings yield different subpopulations of urinary extracellular vesicles from human samples

F Royo, P Zuñiga-Garcia… - Journal of …, 2016 - Taylor & Francis
Urine sample analysis is irreplaceable as a non-invasive method for disease diagnosis and
follow-up. However, in urine samples, non-degraded protein and RNA may be only found in …

Urinary extracellular microvesicles: isolation methods and prospects for urinary proteome

D Wang, W Sun - Proteomics, 2014 - Wiley Online Library
Extracellular microvesicles (EVs) are membranous vesicles, which are released from
diverse cells. These EVs have also been found in a wide range of body fluids. The cargo of …

[HTML][HTML] A simplified method to recover urinary vesicles for clinical applications and sample banking

L Musante, D Tataruch, D Gu, A Benito-Martin… - Scientific reports, 2014 - nature.com
Urinary extracellular vesicles provide a novel source for valuable biomarkers for kidney and
urogenital diseases: Current isolation protocols include laborious, sequential centrifugation …