Development of a scalable single process for producing SARS-CoV-2 RBD monomer and dimer vaccine antigens

T Boggiano-Ayo, J Palacios-Oliva… - … in Bioengineering and …, 2023 - frontiersin.org
We have developed a single process for producing two key COVID-19 vaccine antigens:
SARS-CoV-2 receptor binding domain (RBD) monomer and dimer. These antigens are …

Rapid high-yield production of functional sars-cov-2 receptor binding domain by viral and non-viral transient expression for pre-clinical evaluation

O Farnós, A Venereo-Sánchez, X Xu, C Chan, S Dash… - Vaccines, 2020 - mdpi.com
Vaccine design strategies against severe acute respiratory syndrome coronavirus 2 (SARS-
CoV-2) are focused on the Spike protein or its subunits as the main antigen target of …

Oral administration of a recombinant modified RBD antigen of SARS-CoV-2 as a possible immunostimulant for the care of COVID-19

NA Valdez‑Cruz, D Rosiles-Becerril… - Microbial Cell …, 2024 - Springer
Background Developing effective vaccines against SARS-CoV-2 that consider
manufacturing limitations, equitable access, and acceptance is necessary for developing …

Development of a recombinant RBD subunit vaccine for SARS-CoV-2

YS Sun, JJ Zhou, HP Zhu, F Xu, WB Zhao, HJ Lu… - Viruses, 2021 - mdpi.com
The novel coronavirus pneumonia (COVID-19) pandemic is a great threat to human society
and now is still spreading. Although several vaccines have been authorized for emergency …

Designed SARS‐CoV‐2 receptor binding domain variants form stable monomers

M Klausberger, NF Kienzl, G Stadlmayr… - Biotechnology …, 2022 - Wiley Online Library
The receptor binding domain (RBD) of the SARS‐CoV‐2 spike (S)‐protein is a prime target
of virus‐neutralizing antibodies present in convalescent sera of COVID‐19 patients and thus …

Process development and scale-up optimization of the SARS-CoV-2 receptor binding domain–based vaccine candidate, RBD219-N1C1

J Lee, Z Liu, WH Chen, J Wei, R Kundu… - Applied microbiology …, 2021 - Springer
Abstract A SARS-CoV-2 RBD219-N1C1 (RBD219-N1C1) recombinant protein antigen
formulated on Alhydrogel® has recently been shown to elicit a robust neutralizing antibody …

[HTML][HTML] An engineered SARS-CoV-2 receptor-binding domain produced in Pichia pastoris as a candidate vaccine antigen

M Limonta-Fernández, G Chinea-Santiago… - New …, 2022 - Elsevier
Developing affordable and easily manufactured SARS-CoV-2 vaccines will be essential to
achieve worldwide vaccine coverage and long-term control of the COVID-19 pandemic …

Enhancing the immunogenicity of RBD protein variants through amino acid E484 mutation in SARS-CoV-2

Z Zhang, X Wan, X Li, S Cai, C Wan - Viruses, 2022 - mdpi.com
In the context of the COVID-19 pandemic, conducting antibody testing and vaccination is
critical. In particular, the continued evolution of SARS-CoV-2 raises concerns about the …

[HTML][HTML] Yeast-expressed recombinant SARS-CoV-2 receptor binding domain RBD203-N1 as a COVID-19 protein vaccine candidate

WH Chen, J Pollet, U Strych, J Lee, Z Liu… - Protein Expression and …, 2022 - Elsevier
SARS-CoV-2 protein subunit vaccines are currently being evaluated by multiple
manufacturers to address the global vaccine equity gap, and need for low-cost, easy to …

Enhanced Immunogenicity and Protective Effects against SARS-CoV-2 Following Immunization with a Recombinant RBD-IgG Chimeric Protein

MO Silva, MF Castro-Amarante… - Vaccines, 2024 - mdpi.com
The unprecedented global impact caused by SARS-CoV-2 imposed huge health and
economic challenges, highlighting the urgent need for safe and effective vaccines. The …