CRISPR technology: A decade of genome editing is only the beginning

JY Wang, JA Doudna - Science, 2023 - science.org
The advent of clustered regularly interspaced short palindromic repeat (CRISPR) genome
editing, coupled with advances in computing and imaging capabilities, has initiated a new …

The emerging and uncultivated potential of CRISPR technology in plant science

Y Zhang, AA Malzahn, S Sretenovic, Y Qi - Nature Plants, 2019 - nature.com
The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …

CRISPR/Cas: a Nobel Prize award-winning precise genome editing technology for gene therapy and crop improvement

C Li, E Brant, H Budak, B Zhang - Journal of Zhejiang University-SCIENCE …, 2021 - Springer
Since it was first recognized in bacteria and archaea as a mechanism for innate viral
immunity in the early 2010s, clustered regularly interspaced short palindromic repeats …

CRISPR-based genome editing tools: Insights into technological breakthroughs and future challenges

M Mushtaq, A Ahmad Dar, M Skalicky, A Tyagi… - Genes, 2021 - mdpi.com
Genome-editing (GE) is having a tremendous influence around the globe in the life science
community. Among its versatile uses, the desired modifications of genes, and more …

A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation

LG Lowder, D Zhang, NJ Baltes, JW Paul III… - Plant …, 2015 - academic.oup.com
The relative ease, speed, and biological scope of clustered regularly interspaced short
palindromic repeats (CRISPR)/CRISPR-associated Protein9 (Cas9)-based reagents for …

Targeted gene manipulation in plants using the CRISPR/Cas technology

D Zhang, Z Li, JF Li - Journal of Genetics and Genomics, 2016 - Elsevier
The CRISPR/Cas technology is emerging as a revolutionary genome editing tool in diverse
organisms including plants, and has quickly evolved into a suite of versatile tools for …

Exploring the potential of CRISPR/Cas genome editing for vegetable crop improvement: An overview of challenges and approaches

T Das, U Anand, T Pal, S Mandal… - Biotechnology and …, 2023 - Wiley Online Library
Vegetables provide many nutrients in the form of fiber, vitamins, and minerals, which make
them an important part of our diet. Numerous biotic and abiotic stresses can affect crop …

CRISPR/Cas9 in plants: at play in the genome and at work for crop improvement

B Hussain, SJ Lucas, H Budak - Briefings in functional genomics, 2018 - academic.oup.com
The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9
(CRISPR/Cas9) system uses single-guide RNAs for genome editing, making it a simple …

A technological and regulatory outlook on CRISPR crop editing

R Globus, U Qimron - Journal of cellular biochemistry, 2018 - Wiley Online Library
Generating plants with increased yields while maintaining low production and maintenance
costs is highly important since plants are the major food source for humans and animals, as …

Rapid evolution of manifold CRISPR systems for plant genome editing

L Lowder, A Malzahn, Y Qi - Frontiers in plant science, 2016 - frontiersin.org
Advanced CRISPR-Cas9 based technologies first validated in mammalian cell systems are
quickly being adapted for use in plants. These new technologies increase CRISPR-Cas9's …