Carboxymethyl cellulose and glycerol act synergistically as cryoprotectant during cryopreservation of ram semen

RK Paul, D Kumar, R Singh - Cryobiology, 2021 - Elsevier
Wider implementation of AI in sheep in the field condition has not been possible till date due
to very poor conception rate after cervical insemination with cryopreserved semen. Poor …

Cryopreservation effects on ram sperm ultrastructure

N Keskin, C Erdogan, MN Bucak, AE Ozturk… - Biopreservation and …, 2020 - liebertpub.com
Cryoprotectants are known to have protective effects against cryodamage to spermatozoa. In
this study, the cryoprotective effects of two cryoprotectants (glycerol, ethylene glycol) and …

Current status and advances in ram semen cryopreservation

A Ntemka, IA Tsakmakidis… - Journal of the …, 2018 - ejournals.epublishing.ekt.gr
Ram semen cryopreservation contributes to genetic improvement through artificial
insemination, eliminates geographical barriers in artificial insemination application and …

Effects of low molecular weight cryoprotectants on the post-thaw ram sperm quality and fertilizing ability

S Alcay, B Ustuner, Z Nur - Small Ruminant Research, 2016 - Elsevier
The aim of the current study was to compare the cryoprotective effects of low molecular
weight cryoprotectants 6% DMSO, 6% ethylene glycol, 6% 1, 2 propanediol and 6% glycerol …

Treating ram sperm with cholesterol-loaded cyclodextrins improves cryosurvival

E Mocé, PH Purdy, JK Graham - Animal reproduction science, 2010 - Elsevier
Acceptable fertility using cryopreserved ram sperm is currently only achieved using
laparoscopic intrauterine insemination. Improving the cryosurvival of ram sperm may permit …

Micro-quantity straw as a carrier for cryopreservation of oligozoospermic semen samples: Effects of storage times and cryoprotectant

N Ziarati, TR Topraggaleh, P Rahimizadeh… - Cryobiology, 2019 - Elsevier
Application of an appropriate freezing carrier is crucial for improving post-thaw recovery of
oligozoospermic samples. In this study, our purpose is developing a user-friendly, easy …

Pre-freezing equilibration for 22 h improves post-thaw sperm functions in cryopreserved ram semen by reducing cholesterol efflux

RK Paul, K Balaganur, D Kumar, R Singh - Cryobiology, 2020 - Elsevier
Failure of cervical insemination with cryopreserved semen is hindering implementation of AI
in sheep in field condition. Here the effect of equilibration time and catalase on post-thaw …

Effects of glycerol and sugar mixing temperature on the morphologic and functional integrity of cryopreserved ram sperm

V Pelufo, MFL Armengol, V Malcotti, A Venturino… - Theriogenology, 2015 - Elsevier
Sperm deep freezing procedures for ram semen have considerable variations regarding the
steps being employed for cooling, freezing, and addition of cryoprotectants. In this work, we …

Field and in vitro assay of three methods for freezing ram semen

L Anel, P De Paz, M Alvarez, CA Chamorro, JC Boixo… - Theriogenology, 2003 - Elsevier
Glycerol has been the most widely used cryopreservation agent for spermatozoa and a wide
range of factors affect its action on sperm viability and fertilizing capacity. We tested three …

Dimethylacetamide and trehalose for ram semen cryopreservation

RF Bittencourt, E Oba, CE de Almeida Biscarde… - Cryobiology, 2018 - Elsevier
Eighteen semen samples from nine Santa Ines rams were cryopreserved in order to study
the cryoprotective capacity of dimethylacetamide (DMA) at two concentrations (3% and 6%) …