Detection and Identification of Old World Leishmania by High Resolution Melt Analysis

D Talmi-Frank, A Nasereddin, LF Schnur… - PLoS neglected …, 2010 - journals.plos.org
Background Three major forms of human disease, cutaneous leishmaniasis, visceral
leishmaniasis and mucocutaneous leishmaniasis, are caused by several leishmanial …

Identification of Six New World Leishmania species through the implementation of a High-Resolution Melting (HRM) genotyping assay

C Hernández, C Alvarez, C González, MS Ayala… - Parasites & vectors, 2014 - Springer
Background Leishmaniases are tropical zoonotic diseases, caused by parasites from the
genus Leishmania. New World (NW) species are related to sylvatic cycles although …

Universal PCR assays for the differential detection of all Old World Leishmania species

S Ogado Ceasar Odiwuor, A Ageed Saad… - European journal of …, 2011 - Springer
For the epidemiological monitoring and clinical case management of leishmaniasis,
determination of the causative Leishmania species gains importance. Current assays for the …

Detection and species identification of Old World Leishmania in clinical samples using a PCR-based method

G Spanakos, ET Piperaki, PG Menounos… - Transactions of the …, 2008 - academic.oup.com
The aim of this study was to develop a simple, low-cost method for the detection and species
differentiation of Leishmania directly from clinical samples, for routine use in a parasitology …

Fluorogenic Assay for Molecular Typing of the Leishmania donovani Complex: Taxonomic and Clinical Applications

KW Quispe-Tintaya, T Laurent… - The Journal of …, 2005 - academic.oup.com
We describe a new fluorogenic assay for the identification of species and intraspecies
groups within the Leishmania donovani complex. The assay combined (1) 2 polymerase …

Diagnostic genotyping of Old and New World Leishmania species by PCR-RFLP

J Marfurt, I Niederwieser, ND Makia, HP Beck… - … and infectious disease, 2003 - Elsevier
We have designed a new genotyping scheme for molecular diagnosis of the different
Leishmania species pathogenic to humans. This scheme is based on PCR amplified …

A FRET-based real-time PCR assay to identify the main causal agents of New World tegumentary leishmaniasis

P Tsukayama, JH Núñez… - PLoS neglected …, 2013 - journals.plos.org
In South America, various species of Leishmania are endemic and cause New World
tegumentary leishmaniasis (NWTL). The correct identification of these species is critical for …

Diagnosis and identification of Leishmania spp. from Giemsa-stained slides, by real-time PCR and melting curve analysis in south-west of Iran

S Khademvatan, N Neisi, S Maraghi… - Annals of Tropical …, 2011 - Taylor & Francis
Background: The aim of present study was describing a real-time PCR assay for the
diagnosis and direct identification of Leishmania species on Giemsa-stained slides in south …

First report on natural Leishmania infection of Phlebotomus sergenti due Leishmania tropica by high resolution melting curve method in South-eastern Iran

AA Aghaei, Y Rassi, I Sharifi, H Vatandoost… - Asian Pacific journal of …, 2014 - Elsevier
Abstract Objective To identify the Leishmania species in infected sand flies by Real-time
PCR coupled with HRM analysis. Methods Real-time PCR coupled with HRM analysis …

Diagnostic accuracy of a new Leishmania PCR for clinical visceral leishmaniasis in Nepal and its role in diagnosis of disease

S Deborggraeve, M Boelaert, S Rijal… - Tropical Medicine & …, 2008 - Wiley Online Library
Objective To develop a new PCR for Leishmania detection and to estimate its diagnostic
accuracy in a visceral leishmaniasis (VL) endemic area. Methods After providing the proof …