Visualization of biological processes and pathologic conditions at the cellular and tissue
levels largely relies on the use of fluorescence intensity signals from fluorophores or their …

Fluorescence lifetime imaging (FLIM) uses the fact that the fluorescence lifetime of a
fluorophore depends on its molecular environment but not on its concentration. Molecular …

This article presents an overview of time-resolved (lifetime) fluorescence techniques used in
biomedical diagnostics. In particular, we review the development of time-resolved …

Fluorescence lifetime-resolved imaging (FLI) is a relatively new technique of fluorescence
imaging whereby the spatial distribution of fluorescence decay times can be determined …

Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

This book reflects the remarkable advances in time-resolved fluorescence techniques and
their role in a wide range of biological and clinical applications. Fluorescence lifetime …

Fluorescence lifetime imaging (FLIM) and fluorescence polarization imaging are
complementary techniques that can be used to extract information about macromolecules …

We describe a new fluorescence imaging methodology in which the image contrast is
derived from the fluorescence lifetime at each point in a two-dimensional image and not the …

Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photophysical events that …

Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photo physical events that …