Background Massively parallel pyrosequencing of amplicons from the V6 hypervariable
regions of small-subunit (SSU) ribosomal RNA (rRNA) genes is commonly used to assess …

Initial environmental pyrosequencing studies suggested highly complex protistan
communities with phylotype richness decisively higher than previously estimated. However …

Concerns regarding the rapid loss of endemic biodiversity, and introduction and spread of
non‐indigenous species, have focused attention on the need and ability to detect species …

Massively parallel pyrosequencing of hypervariable regions from small subunit ribosomal
RNA (SSU rRNA) genes can sample a microbial community two or three orders of …

Recent advances in next-generation sequencing (NGS) technologies spur progress in
determining the microbial diversity in various ecosystems by highlighting, for example, the …

Abstract The SRA (Sequence Read Archive) serves as primary depository for massive
amounts of Next Generation Sequencing data and currently host over 100,000 16S rRNA …

High throughput sequencing technology has great promise for biodiversity studies.
However, an underlying assumption is that the primers used in these studies are universal …

Massively parallel pyrosequencing of the small subunit (16S) ribosomal RNA gene has
revealed that the extent of rare microbial populations in several environments, the 'rare …

Small subunit rRNA (SSU rRNA) amplicon sequencing can quantitatively and
comprehensively profile natural microbiomes, representing a critically important tool for …

Next-generation DNA sequencing (NGS) approaches are rapidly surpassing Sanger
sequencing for characterizing the diversity of natural microbial communities. Despite this …