TimeLapse-seq: adding a temporal dimension to RNA sequencing through nucleoside recoding

JA Schofield, EE Duffy, L Kiefer, MC Sullivan… - Nature …, 2018 - nature.com
RNA sequencing (RNA-seq) offers a snapshot of cellular RNA populations, but not temporal
information about the sequenced RNA. Here we report TimeLapse-seq, which uses …

Advancing RNA-seq analysis

BJ Haas, MC Zody - Nature biotechnology, 2010 - nature.com
Advancing RNA-Seq analysis | Nature Biotechnology Skip to main content Thank you for
visiting nature.com. You are using a browser version with limited support for CSS. To obtain the …

Defining transcribed regions using RNA-seq

BT Wilhelm, S Marguerat, I Goodhead, J Bähler - Nature protocols, 2010 - nature.com
Next-generation sequencing technologies are revolutionizing genomics research. It is now
possible to generate gigabase pairs of DNA sequence within a week without time …

RNA timestamps identify the age of single molecules in RNA sequencing

SG Rodriques, LM Chen, S Liu, ED Zhong… - Nature …, 2021 - nature.com
Current approaches to single-cell RNA sequencing (RNA-seq) provide only limited
information about the dynamics of gene expression. Here we present RNA timestamps, a …

Strand-specific libraries for high throughput RNA sequencing (RNA-Seq) prepared without poly (A) selection

Z Zhang, WE Theurkauf, Z Weng, PD Zamore - Silence, 2012 - Springer
Background High throughput DNA sequencing technology has enabled quantification of all
the RNAs in a cell or tissue, a method widely known as RNA sequencing (RNA-Seq) …

Well-TEMP-seq as a microwell-based strategy for massively parallel profiling of single-cell temporal RNA dynamics

S Lin, K Yin, Y Zhang, F Lin, X Chen, X Zeng… - Nature …, 2023 - nature.com
Single-cell RNA sequencing (scRNA-seq) reveals the transcriptional heterogeneity of cells,
but the static snapshots fail to reveal the time-resolved dynamics of transcription. Herein, we …

RNA‐Seq methods for transcriptome analysis

R Hrdlickova, M Toloue, B Tian - Wiley Interdisciplinary …, 2017 - Wiley Online Library
Deep sequencing has been revolutionizing biology and medicine in recent years, providing
single base‐level precision for our understanding of nucleic acid sequences in high …

RNA sequencing and analysis

KR Kukurba, SB Montgomery - Cold Spring Harbor Protocols, 2015 - cshprotocols.cshlp.org
RNA sequencing (RNA-Seq) uses the capabilities of high-throughput sequencing methods
to provide insight into the transcriptome of a cell. Compared to previous Sanger sequencing …

RNA-seq: from technology to biology

S Marguerat, J Bähler - Cellular and molecular life sciences, 2010 - Springer
Next-generation sequencing technologies are now being exploited not only to analyse static
genomes, but also dynamic transcriptomes in an approach termed RNA-seq. Although these …

Current RNA-seq methodology reporting limits reproducibility

J Simoneau, S Dumontier, R Gosselin… - Briefings in …, 2021 - academic.oup.com
Ribonucleic acid sequencing (RNA-seq) identifies and quantifies RNA molecules from a
biological sample. Transformation from raw sequencing data to meaningful gene or isoform …