Discussion of the antibody repertoire usually emphasizes diversity, but a conspicuous
feature of the light chain repertoire is its lack of diversity. The diversity of reported allelic …

The discovery of lymphocytes bearing two light chains in mice carrying self-reactive antibody
transgenes has challenged the “one lymphocyte–one antibody” rule. However, the extent …

We have sequenced the heavy and light chain genes from 365 IgG+ B cells and found that
24 (6.5%) contain somatically introduced insertions or deletions. These insertions and …

In the bone marrow, diversity in the primary antibody repertoire is created by the
combinatorial rearrangement of different gene segments and by the association of different …

Highlights•Receptor editing can lead to the development of dual Ig light chain B cells.•These
cells are referred to as allelically included B cells.•Allelically included B cells coexpress …

The chronic graft-versus-host (cGvH) reaction is a model of induced lupus caused by
alloreactive CD4+ T cells from a Bm-12 mouse in a C57BL/6 recipient. We used this cGvH …

Immunoglobulin gene recombination can result in the assembly of self-reactive antibodies.
Deletion, anergy or receptor editing normally silence B cells that produce these …

We have used homologous recombination in ES cells to engineer B cell-deficient mice that
are incapable of expressing endogenous immunoglobulin heavy and kappa light chain …

Publisher Summary This chapter reviews the essential facts related to the organization of
immunoglobulin genes, V (D) J recombination, and B-cell development. The chapter also …

Mice carrying transgenic rearranged V region genes in their IgH and Igκ loci to encode an
autoreactive specificity direct the emerging autoreactive progenitors into a pre-B cell …