Unambiguous identification of miRNA: target site interactions by different types of ligation reactions
S Grosswendt, A Filipchyk, M Manzano, F Klironomos… - Molecular cell, 2014 - cell.com
To exert regulatory function, miRNAs guide Argonaute (AGO) proteins to partially
complementary sites on target RNAs. Crosslinking and immunoprecipitation (CLIP) assays …
complementary sites on target RNAs. Crosslinking and immunoprecipitation (CLIP) assays …
Mapping the miRNA interactome by cross-linking ligation and sequencing of hybrids (CLASH)
A Helwak, D Tollervey - Nature protocols, 2014 - nature.com
RNA-RNA interactions have critical roles in many cellular processes, but studying them is
difficult and laborious. Here we describe an experimental procedure, termed cross-linking …
difficult and laborious. Here we describe an experimental procedure, termed cross-linking …
miRNA–target chimeras reveal miRNA 3′-end pairing as a major determinant of Argonaute target specificity
Abstract microRNAs (miRNAs) act as sequence-specific guides for Argonaute (AGO)
proteins, which mediate posttranscriptional silencing of target messenger RNAs. Despite …
proteins, which mediate posttranscriptional silencing of target messenger RNAs. Despite …
Genome-wide identification of miRNA targets by PAR-CLIP
miRNAs are short (20–23nt) RNAs that are loaded into proteins of the Argonaute (AGO)
family and guide them to partially complementary target sites on mRNAs, resulting in mRNA …
family and guide them to partially complementary target sites on mRNAs, resulting in mRNA …
Argonaute HITS-CLIP decodes microRNA–mRNA interaction maps
SW Chi, JB Zang, A Mele, RB Darnell - Nature, 2009 - nature.com
MicroRNAs (miRNAs) have critical roles in the regulation of gene expression; however, as
miRNA activity requires base pairing with only 6-8 nucleotides of messenger RNA …
miRNA activity requires base pairing with only 6-8 nucleotides of messenger RNA …
Re‐thinking miRNA‐mRNA interactions: intertwining issues confound target discovery
N Cloonan - Bioessays, 2015 - Wiley Online Library
Despite a library full of literature on miRNA biology, core issues relating to miRNA target
detection, biological effect, and mode of action remain controversial. This essay proposes …
detection, biological effect, and mode of action remain controversial. This essay proposes …
Comprehensive discovery of endogenous Argonaute binding sites in Caenorhabditis elegans
MicroRNAs (miRNAs) regulate gene expression by guiding Argonaute proteins to specific
target mRNA sequences. Identification of bona fide miRNA target sites in animals is …
target mRNA sequences. Identification of bona fide miRNA target sites in animals is …
A biophysical miRNA-mRNA interaction model infers canonical and noncanonical targets
We introduce a biophysical model of miRNA-target interaction and infer its parameters from
Argonaute 2 cross-linking and immunoprecipitation data. We show that a substantial fraction …
Argonaute 2 cross-linking and immunoprecipitation data. We show that a substantial fraction …
An alternative mode of microRNA target recognition
MicroRNAs (miRNAs) regulate mRNA targets through perfect pairing with their seed region
(positions 2–7). Recently, a precise genome-wide map of miRNA interaction sites in mouse …
(positions 2–7). Recently, a precise genome-wide map of miRNA interaction sites in mouse …
miRNA targeting: growing beyond the seed
LB Chipman, AE Pasquinelli - Trends in genetics, 2019 - cell.com
miRNAs are small RNAs that guide Argonaute proteins to specific target mRNAs to repress
their translation and stability. Canonically, miRNA targeting is reliant on base pairing of the …
their translation and stability. Canonically, miRNA targeting is reliant on base pairing of the …