Nanopore sequencing and full genome de novo assembly of human cytomegalovirus TB40/E reveals clonal diversity and structural variations
Background Human cytomegalovirus (HCMV) has a double-stranded DNA genome of
approximately 235 Kbp that is structurally complex including extended GC-rich repeated …
approximately 235 Kbp that is structurally complex including extended GC-rich repeated …
Human cytomegalovirus genomics and transcriptomics through the lens of next-generation sequencing: revision and future challenges
J Martí-Carreras, P Maes - Virus Genes, 2019 - Springer
The human cytomegalovirus (HCMV) genome was sequenced by hierarchical shotgun
almost 30 years ago. Over these years, low and high passaged strains have been …
almost 30 years ago. Over these years, low and high passaged strains have been …
Genomic and functional characteristics of human cytomegalovirus revealed by next-generation sequencing
The complete genome of human cytomegalovirus (HCMV) was elucidated almost 25 years
ago using a traditional cloning and Sanger sequencing approach. Analysis of the genetic …
ago using a traditional cloning and Sanger sequencing approach. Analysis of the genetic …
A method enabling high-throughput sequencing of human cytomegalovirus complete genomes from clinical isolates
S Sijmons, K Thys, M Corthout, E Van Damme… - PLoS …, 2014 - journals.plos.org
Human cytomegalovirus (HCMV) is a ubiquitous virus that can cause serious sequelae in
immunocompromised patients and in the developing fetus. The coding capacity of the 235 …
immunocompromised patients and in the developing fetus. The coding capacity of the 235 …
Long range PCR-based deep sequencing for haplotype determination in mixed HCMV infections
N Brait, B Külekçi, I Goerzer - BMC genomics, 2022 - Springer
Background Short read sequencing has been used extensively to decipher the genome
diversity of human cytomegalovirus (HCMV) strains, but falls short to reveal individual …
diversity of human cytomegalovirus (HCMV) strains, but falls short to reveal individual …
Direct nanopore sequencing of human cytomegalovirus genomes from high-viral-load clinical samples
KK Li, B Lau, NM Suárez, S Camiolo, R Gunson… - Viruses, 2023 - mdpi.com
Nanopore sequencing is becoming increasingly commonplace in clinical settings,
particularly for diagnostic assessments and outbreak investigations, due to its portability, low …
particularly for diagnostic assessments and outbreak investigations, due to its portability, low …
Genome sequences of diverse human cytomegalovirus strains with utility in drug screening and vaccine evaluation
K Corcoran, CJ Sherrod, EF Perkowski… - Genome …, 2017 - Am Soc Microbiol
Cytomegalovirus displays genetic heterogeneity, which has implications for antiviral and
vaccine development. Many studies have focused on laboratory isolates that have been …
vaccine development. Many studies have focused on laboratory isolates that have been …
High-throughput analysis of human cytomegalovirus genome diversity highlights the widespread occurrence of gene-disrupting mutations and pervasive …
S Sijmons, K Thys, M Mbong Ngwese… - Journal of …, 2015 - Am Soc Microbiol
Human cytomegalovirus is a widespread pathogen of major medical importance. It causes
significant morbidity and mortality in immunocompromised individuals, and congenital …
significant morbidity and mortality in immunocompromised individuals, and congenital …
Sequences of complete human cytomegalovirus genomes from infected cell cultures and clinical specimens
C Cunningham, D Gatherer… - Journal of General …, 2010 - microbiologyresearch.org
We have assessed two approaches to sequencing complete human cytomegalovirus
(HCMV) genomes (236 kbp) in DNA extracted from infected cell cultures (strains 3157 …
(HCMV) genomes (236 kbp) in DNA extracted from infected cell cultures (strains 3157 …
Cloning, assembly, and modification of the primary human cytomegalovirus isolate Toledo by yeast-based transformation-associated recombination
S Vashee, TB Stockwell, N Alperovich, EA Denisova… - Msphere, 2017 - Am Soc Microbiol
Genetic engineering of cytomegalovirus (CMV) currently relies on generating a bacterial
artificial chromosome (BAC) by introducing a bacterial origin of replication into the viral …
artificial chromosome (BAC) by introducing a bacterial origin of replication into the viral …