Current ocular microbiome investigations limit reproducibility and reliability: Critical review and opportunities

EM Scott, AC Lewin, ML Leis - Veterinary Ophthalmology, 2021 - Wiley Online Library
Enthusiasm for research describing microbial communities using next‐generation
sequencing (NGS) has outpaced efforts to standardize methodology. Without consistency in …

Veterinary ocular microbiome: Lessons learned beyond the culture

KC Banks, AC Ericsson, CR Reinero… - Veterinary …, 2019 - Wiley Online Library
Ocular pathogens cause many painful and vision‐threatening diseases such as infectious
keratitis, uveitis, and endophthalmitis. While virulent pathogens and pathobionts play …

Next-generation sequencing in ophthalmology: The microbiome in ocular health and disease

H Eguchi, J Ozkan, MJ Holland - Frontiers in Cellular and Infection …, 2022 - frontiersin.org
Application of next-generation sequencing (NGS) technology in infectious diseases has
allowed the simultaneous analysis of genome information from a myriad of microorganisms …

[HTML][HTML] Methodologic Considerations for Studying the Ocular Surface Microbiome

A Chowdhary, RN Van Gelder, M Sundararajan - Ophthalmology Science, 2023 - Elsevier
The ocular surface microbiome, unlike that of the skin or gut, has not been well
characterized. Culture experiments historically suggested a nearly sterile ocular surface, but …

Evaluation of the bacterial ocular surface microbiome in clinically normal horses before and after treatment with topical neomycin-polymyxin-bacitracin

EM Scott, C Arnold, S Dowell, JS Suchodolski - PLoS One, 2019 - journals.plos.org
Next generation sequencing (NGS) studies have demonstrated a rich and diverse ocular
surface-associated microbiota in people that was previously undetected by traditional …

Initial description of the core ocular surface microbiome in dogs: Bacterial community diversity and composition in a defined canine population

ML Leis, MO Costa - Veterinary ophthalmology, 2019 - Wiley Online Library
Objective To characterize the bacterial community residing on the conjunctiva of clinically
healthy dogs. Methods Bacterial DNA from conjunctival swabs of 10 dogs with normal ocular …

Challenges and insights in the exploration of the low abundance human ocular surface microbiome

EL Herzog, M Kreuzer, MS Zinkernagel… - Frontiers in cellular …, 2023 - frontiersin.org
Purpose The low microbial abundance on the ocular surface results in challenges in the
characterization of its microbiome. The purpose of this study was to reveal factors …

Evaluation of the bacterial ocular surface microbiome in ophthalmologically normal dogs prior to and following treatment with topical neomycin-polymyxin-bacitracin

CM Rogers, EM Scott, B Sarawichitr, C Arnold… - PLoS …, 2020 - journals.plos.org
The ocular surface microbiome of veterinary species has not been thoroughly characterized
using molecular-based techniques, such as next generation sequencing (NGS), as the vast …

Current knowledge on the human eye microbiome: a systematic review of available amplicon and metagenomic sequencing data

H Delbeke, S Younas, I Casteels… - Acta …, 2021 - Wiley Online Library
Insights in the ocular surface microbiome are still at an early stage and many more
questions remain unanswered compared with other human‐associated microbial …

The porcine corneal surface bacterial microbiome: A distinctive niche within the ocular surface

ML Leis, GM Madruga, MO Costa - PLoS One, 2021 - journals.plos.org
Purpose The ocular surface microbiome has been described as paucibacterial. Until now,
studies investigating the bacterial community associated with the ocular surface through …