Frontiers and techniques in plant gene regulation
Understanding plant gene regulation has been a priority for generations of plant scientists.
However, due to its complex nature, the regulatory code governing plant gene expression …
However, due to its complex nature, the regulatory code governing plant gene expression …
[HTML][HTML] Understanding plant pathogen interactions using spatial and single-cell technologies
Plants are in contact with diverse pathogens and microorganisms. Intense investigation over
the last 30 years has resulted in the identification of multiple immune receptors in model and …
the last 30 years has resulted in the identification of multiple immune receptors in model and …
[HTML][HTML] Chromatin context-dependent regulation and epigenetic manipulation of prime editing
We set out to exhaustively characterize the impact of the cis-chromatin environment on
prime editing, a precise genome engineering tool. Using a highly sensitive method for …
prime editing, a precise genome engineering tool. Using a highly sensitive method for …
[HTML][HTML] A global view of aging and Alzheimer's pathogenesis-associated cell population dynamics and molecular signatures in human and mouse brains
A Sziraki, Z Lu, J Lee, G Banyai, S Anderson… - Nature Genetics, 2023 - nature.com
Conventional methods fall short in unraveling the dynamics of rare cell types related to
aging and diseases. Here we introduce EasySci, an advanced single-cell combinatorial …
aging and diseases. Here we introduce EasySci, an advanced single-cell combinatorial …
[HTML][HTML] Multiplex profiling of developmental cis-regulatory elements with quantitative single-cell expression reporters
The inability to scalably and precisely measure the activity of developmental cis-regulatory
elements (CREs) in multicellular systems is a bottleneck in genomics. Here we develop a …
elements (CREs) in multicellular systems is a bottleneck in genomics. Here we develop a …
A dynamical perspective: moving towards mechanism in single-cell transcriptomics
RJ Maizels - … Transactions of the Royal Society B, 2024 - royalsocietypublishing.org
As the field of single-cell transcriptomics matures, research is shifting focus from
phenomenological descriptions of cellular phenotypes to a mechanistic understanding of the …
phenomenological descriptions of cellular phenotypes to a mechanistic understanding of the …
Scalable single-cell profiling of chromatin modifications with sciCUT&Tag
DH Janssens, JE Greene, SJ Wu, CA Codomo… - Nature …, 2024 - nature.com
Cleavage under targets and tagmentation (CUT&Tag) is an antibody-directed in situ
chromatin profiling strategy that is rapidly replacing immune precipitation-based methods …
chromatin profiling strategy that is rapidly replacing immune precipitation-based methods …
[HTML][HTML] A single-cell transcriptional timelapse of mouse embryonic development, from gastrula to pup
The house mouse, Mus musculus, is an exceptional model system, combining genetic
tractability with close homology to human biology. Gestation in mouse development lasts …
tractability with close homology to human biology. Gestation in mouse development lasts …
[HTML][HTML] Different effects of CYP27A1 and CYP7B1 on cognitive function: Two mouse models in comparison
J Goikolea, M Latorre-Leal, C Tsagkogianni… - The Journal of steroid …, 2023 - Elsevier
Abstract The oxysterol 27-hydroxycholesterol (27OHC) is produced by the enzyme sterol 27-
hydroxylase (Cyp27A1) and is mainly catabolized to 7α-Hydroxy-3-oxo-4-cholestenoic acid …
hydroxylase (Cyp27A1) and is mainly catabolized to 7α-Hydroxy-3-oxo-4-cholestenoic acid …
[HTML][HTML] Transcriptomic, epigenomic, and spatial metabolomic cell profiling redefines regional human kidney anatomy
A large-scale multimodal atlas that includes major kidney regions is lacking. Here, we
employed simultaneous high-throughput single-cell ATAC/RNA sequencing (SHARE-seq) …
employed simultaneous high-throughput single-cell ATAC/RNA sequencing (SHARE-seq) …