Purification and Characterization of a Novel Thermostable Papain Inhibitor from Moringa oleifera with Antimicrobial and Anticoagulant Properties

J Cotabarren, S Claver, JA Payrol, J Garcia-Pardo… - Pharmaceutics, 2021 - mdpi.com
Plant cystatins (or phytocystatins) comprise a large superfamily of natural bioactive small
proteins that typically act as protein inhibitors of papain-like cysteine proteases. In this …

Molecular Cloning and Characterization of Novel Phytocystatin Gene from Turmeric, Curcuma longa

SN Chan, N Abu Bakar, M Mahmood… - BioMed research …, 2014 - Wiley Online Library
Phytocystatin, a type of protease inhibitor (PI), plays major roles in plant defense
mechanisms and has been reported to show antipathogenic properties and plant stress …

Isolation and purification of phytocystatin from almond: Biochemical, biophysical, and immunological characterization

AA Siddiqui, PSS Khaki, A Sohail, T Sarwar… - Cogent Biology, 2016 - Taylor & Francis
It is well known that fruit nuts contain wide variety of flavonoids and various proteins,
consumption of which has been associated with the reduced risk of chronic diseases …

A new Piper nigrum cysteine proteinase inhibitor, PnCPI, with antifungal activity: molecular cloning, recombinant expression, functional analyses and molecular …

AM Lima, NLF Barros, ACO Freitas, LSC Tavares… - Planta, 2020 - Springer
Main conclusion A new Piper nigrum cysteine proteinase inhibitor, PnCPI, belonging to
group I of phytocystatins, with inhibitory activity against papain and growth of Fusarium …

Purification and biochemical characterization of phytocystatin from Brassica alba

A Ahmed, A Shamsi, B Bano - Journal of Molecular Recognition, 2016 - Wiley Online Library
Phytocystatins belong to the family of cysteine proteinases inhibitors. They are ubiquitously
found in plants and carry out various significant physiological functions. These plant derived …

Crystal structure and physicochemical characterization of a phytocystatin from Humulus lupulus: Insights into its domain-swapped dimer

GT de Moura, AA Souza, AV Garay… - … et Biophysica Acta (BBA …, 2021 - Elsevier
Phytocystatins are a family of plant cysteine-protease inhibitors of great interest due to their
biotechnological application in culture improvement. It was shown that their expression in …

Purification and characterization of a cystatin like thiol protease inhibitor from Brassica nigra

A Feroz, PSS Khaki, AA Siddiqui, F Amin… - International journal of …, 2019 - Elsevier
Phytocystatins or plant cystatins belong to a group of thiol protease inhibitors present
ubiquitously in living system. They play a crucial role in cellular protein turnover thereby …

Insight into the biochemical, kinetic and spectroscopic characterization of garlic (Allium sativum) phytocystatin: Implication for cardiovascular disease

MF Siddiqui, A Ahmed, B Bano - International journal of biological …, 2017 - Elsevier
Phytocystatins are cysteine proteinase inhibitors present in plants. They play crucial role in
maintaining protease-anti protease balance and are involved in various endogenous …

Physico-chemical and in-silico analysis of a phytocystatin purified from Brassica juncea cultivar RoAgro 5444

S Khan, S Ahmad, MI Siddiqi… - Biochemistry and Cell …, 2016 - cdnsciencepub.com
This study describes the isolation and purification of a phytocystatin from seeds of Brassica
juncea (Indian mustard; cultivar RoAgro 5444), which is an important oilseed crop both …

Insight into the biochemical characterization of phytocystatin from Glycine max and its interaction with Cd+2 and Ni+2

S Siddiqui, MF Siddiqui, S Khan… - Journal of Molecular …, 2019 - Wiley Online Library
Phytocystatins are cysteine proteinase inhibitors ubiquitously present in plants and animals.
They are known to carry out various significant physiological functions and also maintain the …