The complex cellular heterogeneity of the lung poses a unique challenge to researchers in
the field. While the use of bulk RNA sequencing has become a ubiquitous technology in …

Lung development is a complicated and delicate process, facilitated by spatially and
temporarily coordinated cross talk of up to 40 cell types. Developmental origin and …

The past four decades have yielded advances in molecular biology allowing detailed
characterization of the cellular genome and the transcriptome: the complete set of RNA …

Single-cell RNA sequencing (scRNASeq) has advanced our understanding of lung biology,
but its utility is limited by the need for fresh samples, loss of cell types by death or …

Precision-cut lung slices (PCLS) have gained increasing interest as a model to study lung
biology/disease and screening novel therapeutics. In particular, PCLS derived from human …

The respiratory system is a vital component of our body, essential for both oxygen uptake
and immune defense. Knowledge of cellular composition and function in different parts of …

Pulmonary fibrosis is a devastating disorder that results in the progressive replacement of
normal lung tissue with fibrotic scar. Available therapies slow disease progression, but most …

Single-cell ribonucleic acid sequencing is becoming widely employed to study biological
processes at a novel resolution depth. The ability to analyse transcriptomes of multiple …

Editorial summary Single-cell transcriptome profiling has enabled high-resolution analysis of
cellular populations in tissues during development, health, and disease. Recent studies …

Single-cell RNA sequencing (scRNA-seq) is an important tool for understanding disease
pathophysiology, including airway diseases. Currently, the majority of scRNA-seq studies in …