Abstract In three‐dimensional (3D) space, an unbiased and systemic view of human
specimens between structures and functions is required. However, conventional histological …

Organisms are inherently three dimensional, thus comprehensive understanding of the
complicated biological system requires analysis of organs or even whole bodies in the …

Tissue clearing of gross anatomical samples was first described more than a century ago
and has only recently found widespread use in the field of microscopy. This renaissance has …

Tissue clearing has become a powerful technique for studying anatomy and morphology at
scales ranging from entire organisms to subcellular features. With the recent proliferation of …

The last decade has seen a proliferation of tissue clearing methods that render large
biological samples transparent and allow unprecedented three-dimensional views of …

State-of-the-art tissue-clearing methods provide subcellular-level optical access to intact
tissues from individual organs and even to some entire mammals. When combined with light …

Chemical clearing of fixed tissues is becoming a key instrument for the three-dimensional
reconstruction of macroscopic tissue portions, including entire organs. Indeed, the growing …

The recent growth of tissue clearing technology supports the dissemination and
generalization of three-dimensional (3D) observations of complex biological tissues. Since …

The understanding of spatially complex biological systems is greatly aided by the availability
of high resolution information on their tissue architecture, as is provided by optical sectioning …

Tissue clearing has become an important tool for the investigation of biological systems in
three dimensions. However, many pioneering techniques were based on serendipitous …