Cleavage under targets and tagmentation (CUT&Tag) is an antibody-directed in situ
chromatin profiling strategy that is rapidly replacing immune precipitation-based methods …

Methods for quantifying gene expression and chromatin accessibility in single cells are well
established, but single-cell analysis of chromatin regions with specific histone modifications …

Many chromatin features play critical roles in regulating gene expression. A complete
understanding of gene regulation will require the mapping of specific chromatin features in …

In contrast to single-cell approaches for measuring gene expression and DNA accessibility,
single-cell methods for analyzing histone modifications are limited by low sensitivity and …

Epigenetic mechanisms such as histone modifications play critical roles in adaptive tuning of
chromatin structures. Profiling of various histone modifications at the genome scale using …

Chromatin profiling in single cells has been extremely challenging and almost exclusively
limited to histone proteins. In cases where single-cell methods have shown promise, many …

Probing histone modifications at a single-cell level in thousands of cells has been enabled
by technologies such as single-cell CUT&Tag. Here we describe nano-CUT&Tag (nano-CT) …

Abstract We recently introduced Cleavage Under Targets & Tagmentation (CUT&Tag), an
epigenomic profiling strategy in which antibodies are bound to chromatin proteins in situ in …

The ability to comprehensively analyze the chromatin state with single-cell resolution is
crucial for understanding gene regulatory principles in heterogenous tissues or during …

Genome-wide location analysis of histone modifications and transcription factor binding
relies on chromatin immunoprecipitation (ChIP) assays. These assays are, however, time …