Natural and synthetic biomaterials for engineering multicellular tumor spheroids
The lack of in vitro models that represent the native tumor microenvironment is a significant
challenge for cancer research. Two-dimensional (2D) monolayer culture has long been the …
challenge for cancer research. Two-dimensional (2D) monolayer culture has long been the …
Engineering a scaffold-free 3D tumor model for in vitro drug penetration studies
Three-dimensional (3D) in vitro cultures are recognized for recapitulating the physiological
microenvironment and exhibiting high concordance with in vivo conditions. In cancer …
microenvironment and exhibiting high concordance with in vivo conditions. In cancer …
Comparative study of multicellular tumor spheroid formation methods and implications for drug screening
MF Gencoglu, LE Barney, CL Hall… - ACS biomaterials …, 2018 - ACS Publications
Improved in vitro models are needed to better understand cancer progression and bridge
the gap between in vitro proof-of-concept studies, in vivo validation, and clinical application …
the gap between in vitro proof-of-concept studies, in vivo validation, and clinical application …
Three-dimensional culture systems in cancer research: Focus on tumor spheroid model
Cancer cells propagated in three-dimensional (3D) culture systems exhibit physiologically
relevant cell–cell and cell–matrix interactions, gene expression and signaling pathway …
relevant cell–cell and cell–matrix interactions, gene expression and signaling pathway …
Engineering tumors: a tissue engineering perspective in cancer biology
There is increasing recognition that three-dimensional (3D) tissue culture technologies have
many uses within the biomedical sciences beyond the scope of regenerative medicine. One …
many uses within the biomedical sciences beyond the scope of regenerative medicine. One …
Three-dimensional in vitro culture models in oncology research
Cancer is a multifactorial disease that is responsible for 10 million deaths per year. The intra-
and inter-heterogeneity of malignant tumors make it difficult to develop single targeted …
and inter-heterogeneity of malignant tumors make it difficult to develop single targeted …
Heralding a new paradigm in 3D tumor modeling
Numerous studies to date have contributed to a paradigm shift in modeling cancer, moving
from the traditional two-dimensional culture system to three-dimensional (3D) culture …
from the traditional two-dimensional culture system to three-dimensional (3D) culture …
Generation of multicellular tumor spheroids with microwell-based agarose scaffolds for drug testing
X Gong, C Lin, J Cheng, J Su, H Zhao, T Liu, X Wen… - PloS one, 2015 - journals.plos.org
Three dimensional multicellular aggregate, also referred to as cell spheroid or microtissue,
is an indispensable tool for in vitro evaluating antitumor activity and drug efficacy. Compared …
is an indispensable tool for in vitro evaluating antitumor activity and drug efficacy. Compared …
Challenges of applying multicellular tumor spheroids in preclinical phase
SJ Han, S Kwon, KS Kim - Cancer cell international, 2021 - Springer
Abstract The three-dimensional (3D) multicellular tumor spheroids (MCTs) model is
becoming an essential tool in cancer research as it expresses an intermediate complexity …
becoming an essential tool in cancer research as it expresses an intermediate complexity …
Acquisition of epithelial–mesenchymal transition and cancer stem-like phenotypes within chitosan-hyaluronan membrane-derived 3D tumor spheroids
YJ Huang, S Hsu - Biomaterials, 2014 - Elsevier
Cancer drug development has to go through rigorous testing and evaluation processes
during pre-clinical in vitro studies. However, the conventional two-dimensional (2D) in vitro …
during pre-clinical in vitro studies. However, the conventional two-dimensional (2D) in vitro …