Ion-exchange chromatography for the characterization of biopharmaceuticals

S Fekete, A Beck, JL Veuthey, D Guillarme - Journal of pharmaceutical and …, 2015 - Elsevier
Ion-exchange chromatography (IEX) is a historical technique widely used for the detailed
characterization of therapeutic proteins and can be considered as a reference and powerful …

Core-shell particles lead the way to renewing high-performance liquid chromatography

V González-Ruiz, AI Olives, MA Martín - TrAC Trends in Analytical …, 2015 - Elsevier
In this review, we present the latest highlights and trends in the use of core-shell particles as
stationary phases in columns for liquid chromatography (LC). These highly homogeneous …

Applications of hydrophilic interaction chromatography to amino acids, peptides, and proteins

A Periat, IS Krull, D Guillarme - Journal of separation science, 2015 - Wiley Online Library
This review summarizes the recent advances in the analysis of amino acids, peptides, and
proteins using hydrophilic interaction chromatography. Various reports demonstrate the …

Submicrometer particles and slip flow in liquid chromatography

BA Rogers, Z Wu, B Wei, X Zhang, X Cao, O Alabi… - 2015 - ACS Publications
Smaller particles have progressively led to higher efficiency in liquid chromatography,
particularly for proteins, due to smaller diffusion distances. Particle diameter has recently …

Synthesis and optimization of wide pore superficially porous particles by a one-step coating process for separation of proteins and monoclonal antibodies

W Chen, K Jiang, A Mack, B Sachok, X Zhu… - … of Chromatography A, 2015 - Elsevier
Superficially porous particles (SPPs) with pore size ranging from 90 Å to 120 Å have been a
great success for the fast separation of small molecules over totally porous particles in …

Systematic evaluation of mobile phase additives for the LC–MS characterization of therapeutic proteins

B Bobály, A Beck, J Fekete, D Guillarme, S Fekete - Talanta, 2015 - Elsevier
Trifluoroacetic acid (TFA) is commonly used as mobile phase additive for the analysis of
proteins in reversed phase liquid chromatography (RPLC). Due to its interesting features, it …

Silica, hybrid silica, hydride silica and non-silica stationary phases for liquid chromatography. Part II: chemical and thermal stability

EM Borges, DA Volmer - Journal of chromatographic science, 2015 - academic.oup.com
In the first part of this review, stationary phases (silica, hybrid silica, hydride silica and non-
silica stationary phases) were characterized and compared with respect to selectivity …

[HTML][HTML] Current state of superficially porous particle technology in liquid chromatography

D Bell - 2015 - chromatographyonline.com
The use of superficially porous particles in the manufacture of high performance liquid
chromatography (HPLC) columns has become prominent in recent years. Over the course of …

Evaluation of a new wide-pore superficially porous material with carbon core and nanodiamond-polymer shell for the separation of proteins

S Fekete, DS Jensen, J Zukowski… - Journal of Chromatography …, 2015 - Elsevier
In this study, reversed phase liquid chromatographic columns packed with superficially
porous material made of a carbon core and nanodiamond-polymer shell were evaluated for …

Open tubular capillary column for the separation of cytochrome C tryptic digest in capillary electrochromatography

F Ali, WJ Cheong - Journal of separation science, 2015 - Wiley Online Library
A silica capillary of 50 μm internal diameter and 500 mm length (416 mm effective length)
was chemically modified with 4‐(trifluoromethoxy) phenyl isocyanate in the presence of …