Integrating enzyme immobilization and protein engineering: An alternative path for the development of novel and improved industrial biocatalysts

C Bernal, K Rodriguez, R Martínez - Biotechnology Advances, 2018 - Elsevier
Enzyme immobilization often achieves reusable biocatalysts with improved operational
stability and solvent resistance. However, these modifications are generally associated with …

Thermostable marine microbial proteases for industrial applications: scopes and risks

N Barzkar, A Homaei, R Hemmati, S Patel - Extremophiles, 2018 - Springer
Thermostable proteases are important in biotechnological and industrial sectors, due to their
stability against denaturing agents and chemicals. The feature that gives them such unique …

Investigating the effect of two-point surface attachment on enzyme stability and activity

X Zou, S Wei, S Badieyan, MK Schroeder… - Journal of the …, 2018 - ACS Publications
Immobilization on solid supports provides an effective way to improve enzyme stability and
simplify downstream processing for biotechnological applications, which has been widely …

Influence of Glutaraldehyde Cross-Linking Modes on the Recyclability of Immobilized Lipase B from Candida antarctica for Transesterification of Soy Bean Oil

I A. Modenez, DE Sastre, F C. Moraes… - Molecules, 2018 - mdpi.com
Lipase B from Candida antarctica (CAL-B) is largely employed as a biocatalyst for
hydrolysis, esterification, and transesterification reactions. CAL-B is a good model enzyme to …

Covalently immobilized Trp60Cys mutant of ω-transaminase from Chromobacterium violaceum for kinetic resolution of racemic amines in batch and continuous-flow …

E Abaházi, P Sátorhelyi, B Erdélyi, BG Vértessy… - Biochemical …, 2018 - Elsevier
Covalent immobilization of an engineered ω-transaminase mutant Trp60Cys from
Chromobacterium violaceum (CvTA W60C) was performed on bisepoxide-activated …

Smart nanoparticles for selective immobilization of acid phosphatases

F Nagy, G Tasnádi, D Balogh‐Weiser, E Bell… - …, 2018 - Wiley Online Library
An easy to use method combining the selectivity of metal chelate affinity binding with strong
covalent linking was developed for immobilization of non‐specific acid phosphatases …

Synthesis and chromatographic evaluation of phenyl/tetrazole bonded stationary phase based on thiol‐epoxy ring opening reaction

G Jin, Y Liu, F Yang, D Yu, J Yan… - Journal of separation …, 2018 - Wiley Online Library
A silica‐based reversed‐phase stationary phase bonding with phenyl and tetrazole groups
was synthesized by thiol‐epoxy ring opening reaction. The bonded groups could not only …

Biotransformation of disperse dyes using nitroreductase immobilized on magnetic particles modified with tosyl group: Identification of products by LC-MS-MS and …

JH Franco, BF da Silva, AA de Castro… - Environmental …, 2018 - Elsevier
The present work evaluates the action of nitroreductase enzyme immobilized on
Tosylactivated magnetic particles (MP-Tosyl) on three disperse dyes which contain nitro and …