Here, we describe single-tube long fragment read (stLFR), a technology that enables
sequencing of data from long DNA molecules using economical second-generation …

The development and innovation of next generation sequencing (NGS) and the subsequent
analysis tools have gain popularity in scientific researches and clinical diagnostic …

Single-cell RNA-seq technologies require library preparation prior to sequencing. Here, we
present the first report to compare the cheaper BGISEQ-500 platform to the Illumina HiSeq …

Background RNA-Seq data is inherently nonuniform for different transcripts because of
differences in gene expression. This makes it challenging to decide how much data should …

Currently, Illumina sequencers are the globally leading sequencing platform in the next-
generation sequencing market. Recently, MGI Tech launched a series of new sequencers …

The parathyroid hormone 1 receptor (PTH1R) mediates the biologic actions of parathyroid
hormone (PTH) and parathyroid hormone–related protein (PTHrP). Here, we showed that …

MicroRNAs (miRNAs) hold great promise as blood-borne and circulating biomarkers for
numerous diseases. However, the reliability of such liquid biopsies is particularly impacted …

Droplet microfluidics-based platform (Drop-seq) has been shown to be a powerful tool for
single cell expression profiling. Nevertheless, this platform required the simultaneous …

Aberrant differentiations of bone mesenchymal stem cells (BMSCs) have proved to be
associated with the occurrence of senile osteoporosis. However, mechanisms of this …

Abstract Background Chinese wild grapevine (Vitis amurensis) has remarkable cold stress
tolerance, exceeding that of the common cultivated grapevine (Vitis vinifera L.). Result Here …