Directed evolution: methodologies and applications

Y Wang, P Xue, M Cao, T Yu, ST Lane… - Chemical reviews, 2021 - ACS Publications
Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …

Guidelines for the use of flow cytometry and cell sorting in immunological studies

A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2021 - Wiley Online Library
The third edition of Flow Cytometry Guidelines provides the key aspects to consider when
performing flow cytometry experiments and includes comprehensive sections describing …

Treg-expressed CTLA-4 depletes CD80/CD86 by trogocytosis, releasing free PD-L1 on antigen-presenting cells

M Tekguc, JB Wing, M Osaki, J Long… - Proceedings of the …, 2021 - National Acad Sciences
Foxp3-expressing CD4+ CD25+ regulatory T cells (Tregs) constitutively and highly express
the immune checkpoint receptor cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4) …

[HTML][HTML] SARS-CoV-2–associated ssRNAs activate inflammation and immunity via TLR7/8

V Salvi, HO Nguyen, F Sozio, T Schioppa, C Gaudenzi… - JCI insight, 2021 - ncbi.nlm.nih.gov
The inflammatory and IFN pathways of innate immunity play a key role in the resistance and
pathogenesis of coronavirus disease 2019 (COVID-19). Innate sensors and SARS-CoV-2 …

[HTML][HTML] A binary Cre transgenic approach dissects microglia and CNS border-associated macrophages

JS Kim, M Kolesnikov, S Peled-Hajaj, I Scheyltjens… - Immunity, 2021 - cell.com
The developmental and molecular heterogeneity of tissue macrophages is unravelling, as
are their diverse contributions to physiology and pathophysiology. Moreover, also given …

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1

DJ Klionsky, AK Abdel-Aziz, S Abdelfatah, M Abdellatif… - autophagy, 2021 - Taylor & Francis
In 2008, we published the first set of guidelines for standardizing research in autophagy.
Since then, this topic has received increasing attention, and many scientists have entered …

[HTML][HTML] T cell assays differentiate clinical and subclinical SARS-CoV-2 infections from cross-reactive antiviral responses

A Ogbe, B Kronsteiner, DT Skelly, M Pace… - Nature …, 2021 - nature.com
Identification of protective T cell responses against SARS-CoV-2 requires distinguishing
people infected with SARS-CoV-2 from those with cross-reactive immunity to other …

[HTML][HTML] Endothelial dysfunction contributes to severe COVID-19 in combination with dysregulated lymphocyte responses and cytokine networks

L Ruhl, I Pink, JF Kühne, K Beushausen… - Signal transduction and …, 2021 - nature.com
The systemic processes involved in the manifestation of life-threatening COVID-19 and in
disease recovery are still incompletely understood, despite investigations focusing on the …

Plasmonic optical tweezers for particle manipulation: principles, methods, and applications

Y Ren, Q Chen, M He, X Zhang, H Qi, Y Yan - ACS nano, 2021 - ACS Publications
Inspired by the idea of combining conventional optical tweezers with plasmonic
nanostructures, a technique named plasmonic optical tweezers (POT) has been widely …

Flow cytometry: principles, applications and recent advances

SM Manohar, P Shah, A Nair - Bioanalysis, 2021 - Taylor & Francis
Flow cytometry (FCM) is a sophisticated technique that works on the principle of light
scattering and fluorescence emission by the specific fluorescent probe-labeled cells as they …