Single-cell atlases promise to provide a 'missing link'between genes, diseases and
therapies. By identifying the specific cell types, states, programs and contexts where disease …

Genomic analyses in cancer have been enormously impactful, leading to the identification of
driver mutations and development of targeted therapies. But the functions of the vast majority …

To chart cell composition and cell state changes that occur during the transformation of
healthy colon to precancerous adenomas to colorectal cancer (CRC), we generated single …

A principal challenge in the analysis of tissue imaging data is cell segmentation—the task of
identifying the precise boundary of every cell in an image. To address this problem we …

Pancreatic ductal adenocarcinoma is a lethal disease with limited treatment options and
poor survival. We studied 83 spatial samples from 31 patients (11 treatment-naïve and 20 …

CD8+ T cells specific for cancer cells are detected within tumours. However, despite their
presence, tumours progress. The clinical success of immune checkpoint blockade and …

Methods for profiling genes at the single-cell level have revolutionized our ability to study
several biological processes and systems including development, differentiation, response …

Highly multiplexed tissue imaging makes detailed molecular analysis of single cells possible
in a preserved spatial context. However, reproducible analysis of large multichannel images …

The immune system is a complex, dynamic, and plastic ecosystem composed of multiple cell
types that constantly sense and interact with their local microenvironment to protect from …

Immune-checkpoint inhibitors (ICIs) have transformed patient care in oncology but are
associated with a unique spectrum of organ-specific inflammatory toxicities known as …