Many systems have been designed for the detection of SARS-CoV-2, which is the virus that
causes COVID-19. SARS-CoV-2 is readily transmitted, resulting in the rapid spread of …

This paper offers an exhaustive comparison of quantitative PCR (qPCR) and digital PCR
(dPCR) technologies, focusing on their critical roles in molecular diagnostics, ranging from …

Lateral flow immunoassay (LFIA) has played a vital role in point-of-care (POC) testing on
account of its simplicity, rapidity, and low cost. However, the low sensitivity and difficulty of …

Despite the great potential of CRISPR-based detection, it has not been competitive with
other market diagnostics for on-site and in-home testing. Here we dissect the rate-limiting …

Wastewater-based epidemiology (WBE) has been used for monitoring infectious diseases
like polio, hepatitis, etc. since the 1940s. It is also being used for tracking the SARS-CoV-2 at …

Surface-enhanced Raman scattering (SERS) technique with high sensitivity, reliable
specificity, and rapid recognition ability exhibits attractive promise for the effective fast …

SARS-CoV-2 has been causing severe impacts on all aspects of society for more than three
years. Antigen immunoassay is the main detection method of SARS-CoV-2 at present. Here …

Advancing expeditious and highly responsive in vitro diagnostic techniques is paramount to
ensure the efficacy of disease prevention and control measures. Although polymerase chain …

Nucleic acids are essential biomarkers in molecular diagnostics. The CRISPR/Cas system
has been widely used for nucleic acid detection. Moreover, canonical CRISPR/Cas12a …

How to realize fM-level detection of viral DNA without target amplification is a challenge.
Traditionally, preamplification of low-abundant DNA targets is a prerequisite, which not only …