Directed evolution: methodologies and applications
Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …
and systems in a test tube through iterative rounds of gene diversifications and library …
A compendium of single extracellular vesicle flow cytometry
JA Welsh, GJA Arkesteijn, M Bremer… - Journal of …, 2023 - Wiley Online Library
Flow cytometry (FCM) offers a multiparametric technology capable of characterizing single
extracellular vesicles (EVs). However, most flow cytometers are designed to detect cells …
extracellular vesicles (EVs). However, most flow cytometers are designed to detect cells …
High-speed fluorescence image–enabled cell sorting
Fast and selective isolation of single cells with unique spatial and morphological traits
remains a technical challenge. Here, we address this by establishing high-speed image …
remains a technical challenge. Here, we address this by establishing high-speed image …
Marked T cell activation, senescence, exhaustion and skewing towards TH17 in patients with COVID-19 pneumonia
S De Biasi, M Meschiari, L Gibellini, C Bellinazzi… - Nature …, 2020 - nature.com
The immune system of patients infected by SARS-CoV-2 is severely impaired. Detailed
investigation of T cells and cytokine production in patients affected by COVID-19 pneumonia …
investigation of T cells and cytokine production in patients affected by COVID-19 pneumonia …
Impaired immune cell cytotoxicity in severe COVID-19 is IL-6 dependent
BACKGROUND Coronavirus disease 19 (COVID-19) is an emerging infectious disease
caused by SARS-CoV-2. Antiviral immune response is crucial to achieve pathogen …
caused by SARS-CoV-2. Antiviral immune response is crucial to achieve pathogen …
Meta-analysis of leukocyte diversity in atherosclerotic mouse aortas
The diverse leukocyte infiltrate in atherosclerotic mouse aortas was recently analyzed in 9
single-cell RNA sequencing and 2 mass cytometry studies. In a comprehensive meta …
single-cell RNA sequencing and 2 mass cytometry studies. In a comprehensive meta …
Precise reconstruction of the TME using bulk RNA-seq and a machine learning algorithm trained on artificial transcriptomes
A Zaitsev, M Chelushkin, D Dyikanov, I Cheremushkin… - Cancer Cell, 2022 - cell.com
Cellular deconvolution algorithms virtually reconstruct tissue composition by analyzing the
gene expression of complex tissues. We present the decision tree machine learning …
gene expression of complex tissues. We present the decision tree machine learning …
Altered bioenergetics and mitochondrial dysfunction of monocytes in patients with COVID‐19 pneumonia
L Gibellini, S De Biasi, A Paolini, R Borella… - EMBO molecular …, 2020 - embopress.org
In patients infected by SARS‐CoV‐2 who experience an exaggerated inflammation leading
to pneumonia, monocytes likely play a major role but have received poor attention. Thus, we …
to pneumonia, monocytes likely play a major role but have received poor attention. Thus, we …
Treg-expressed CTLA-4 depletes CD80/CD86 by trogocytosis, releasing free PD-L1 on antigen-presenting cells
Foxp3-expressing CD4+ CD25+ regulatory T cells (Tregs) constitutively and highly express
the immune checkpoint receptor cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4) …
the immune checkpoint receptor cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4) …
[HTML][HTML] SARS-CoV-2–associated ssRNAs activate inflammation and immunity via TLR7/8
V Salvi, HO Nguyen, F Sozio, T Schioppa, C Gaudenzi… - JCI insight, 2021 - ncbi.nlm.nih.gov
The inflammatory and IFN pathways of innate immunity play a key role in the resistance and
pathogenesis of coronavirus disease 2019 (COVID-19). Innate sensors and SARS-CoV-2 …
pathogenesis of coronavirus disease 2019 (COVID-19). Innate sensors and SARS-CoV-2 …