High-throughput and single-cell T cell receptor sequencing technologies
JA Pai, AT Satpathy - Nature methods, 2021 - nature.com
T cells express T cell receptors (TCRs) composed of somatically recombined TCRα and
TCRβ chains, which mediate recognition of major histocompatibility complex (MHC)–antigen …
TCRβ chains, which mediate recognition of major histocompatibility complex (MHC)–antigen …
Adaptive immune receptor repertoire analysis
B cell and T cell receptor repertoires compose the adaptive immune receptor repertoire
(AIRR) of an individual. The AIRR is a unique collection of antigen-specific receptors that …
(AIRR) of an individual. The AIRR is a unique collection of antigen-specific receptors that …
Spatial transcriptomics of B cell and T cell receptors reveals lymphocyte clonal dynamics
The spatial distribution of lymphocyte clones within tissues is critical to their development,
selection, and expansion. We have developed spatial transcriptomics of variable, diversity …
selection, and expansion. We have developed spatial transcriptomics of variable, diversity …
[HTML][HTML] Dandelion uses the single-cell adaptive immune receptor repertoire to explore lymphocyte developmental origins
Assessment of single-cell gene expression (single-cell RNA sequencing) and adaptive
immune receptor (AIR) sequencing (scVDJ-seq) has been invaluable in studying …
immune receptor (AIR) sequencing (scVDJ-seq) has been invaluable in studying …
Benchmarking of T cell receptor repertoire profiling methods reveals large systematic biases
Monitoring the T cell receptor (TCR) repertoire in health and disease can provide key
insights into adaptive immune responses, but the accuracy of current TCR sequencing …
insights into adaptive immune responses, but the accuracy of current TCR sequencing …
Scirpy: a Scanpy extension for analyzing single-cell T-cell receptor-sequencing data
Advances in single-cell technologies have enabled the investigation of T-cell phenotypes
and repertoires at unprecedented resolution and scale. Bioinformatic methods for the …
and repertoires at unprecedented resolution and scale. Bioinformatic methods for the …
The immuneML ecosystem for machine learning analysis of adaptive immune receptor repertoires
Adaptive immune receptor repertoires (AIRR) are key targets for biomedical research as
they record past and ongoing adaptive immune responses. The capacity of machine …
they record past and ongoing adaptive immune responses. The capacity of machine …
[HTML][HTML] Partial RAG deficiency in humans induces dysregulated peripheral lymphocyte development and humoral tolerance defect with accumulation of T-bet+ B …
K Csomos, B Ujhazi, P Blazso, JL Herrera… - Nature …, 2022 - nature.com
The recombination-activating genes (RAG) 1 and 2 are indispensable for diversifying the
primary B cell receptor repertoire and pruning self-reactive clones via receptor editing in the …
primary B cell receptor repertoire and pruning self-reactive clones via receptor editing in the …
B cell phylogenetics in the single cell era
KB Hoehn, SH Kleinstein - Trends in Immunology, 2024 - cell.com
The widespread availability of single-cell RNA sequencing (scRNA-seq) has led to the
development of new methods for understanding immune responses. Single-cell …
development of new methods for understanding immune responses. Single-cell …
Vaccine priming of rare HIV broadly neutralizing antibody precursors in nonhuman primates
JM Steichen, I Phung, E Salcedo, G Ozorowski… - Science, 2024 - science.org
Germline-targeting immunogens hold promise for initiating the induction of broadly
neutralizing antibodies (bnAbs) to HIV and other pathogens. However, antibody-antigen …
neutralizing antibodies (bnAbs) to HIV and other pathogens. However, antibody-antigen …