Zinc-finger nucleases (ZFNs) are powerful tools for experimental gene manipulation. A
number of recent papers have shown how this technology can be applied effectively to …

Genome editing with engineered nucleases is a rapidly growing field thanks to
transformative technologies that allow researchers to precisely alter genomes for numerous …

An efficient method for making directed DNA sequence modifications to plant genes (gene
targeting) is at present lacking, thereby frustrating efforts to dissect plant gene function and …

Custom-made zinc-finger nucleases (ZFNs) can induce targeted genome modifications with
high efficiency in cell types including Drosophila, C. elegans, plants, and humans. A …

Custom-designed nucleases (CDNs) greatly facilitate genetic engineering by generating a
targeted DNA double-strand break (DSB) in the genome. Once a DSB is created, specific …

We report here homologous recombination (HR)-mediated gene targeting of two different
genes in human iPS cells (hiPSCs) and human ES cells (hESCs). HR-mediated correction of …

Recessive dystrophic epidermolysis bullosa (RDEB) is characterized by a functional deficit
of type VII collagen protein due to gene defects in the type VII collagen gene (COL7A1) …

Abstract β-Thalassemia is one of the most common genetic blood diseases and is caused by
either point mutations or deletions in the β-globin (HBB) gene. The generation of patient …

The precise genetic manipulation of stem and precursor cells offers extraordinary potential
for the analysis, prevention, and treatment of human malignancies. Chromosomal …

Engineered zinc-finger nucleases (ZFNs) form the basis of a broadly applicable method for
targeted, efficient modification of eukaryotic genomes. In recent work, we described OPEN …