Precursor messenger RNA (pre-mRNA) splicing is an essential step in the flow of
information from DNA to protein in all eukaryotes. Research over the past four decades has …

). Although introns needed to be removed with single-nucleotide precision to avoid
introducing catastrophic errors of frame, they contained few conserved sequences, varied …

Intermolecular RNA-RNA interactions are used by many noncoding RNAs (ncRNAs) to
achieve their diverse functions. To identify these contacts, we developed a method based on …

A total of 101 different examples of point mutations, which lie in the vicinity of mRNA splice
junctions, and which have been held to be responsible for a human genetic disease by …

To initiate cotranscriptional splicing, RNA polymerase II (Pol II) recruits the U1 small nuclear
ribonucleoprotein particle (U1 snRNP) to nascent precursor messenger RNA (pre-mRNA) …

Artificial neural networks have been applied to the prediction of splice site location in human
pre-mRNA. A joint prediction scheme where prediction of transition regions between introns …

A simple and efficient method for synthesizing pure single stranded RNAs of virtually any
structure is described. This in vitro transcription system is based on the unusually specific …

The removal of intervening sequences from transcripts is catalyzed by the spliceosome, a
multicomponent complex that assembles on the newly synthesized pre-mRNA. Pre-mRNA …

Precursor messenger RNA (pre-mRNA) splicing is executed by the spliceosome. In the past
3 years, cryoelectron microscopy (cryo-EM) structures have been elucidated for a majority of …

Based on ultrastructural analysis of actively transcribing genes seen in electron
micrographs, we present evidence that pre-mRNA splicing occurs with a reasonable …