Fluorescence microscopy is an invaluable tool in the biosciences, a genuine workhorse
technique offering exceptional contrast in conjunction with high specificity of labelling with …

Single-molecule biophysics has transformed our understanding of biology, but also of the
physics of life. More exotic than simple soft matter, biomatter lives far from thermal …

Although not laying claim to being the inventor of the light microscope, Antonj van
Leeuwenhoek (1632–1723) was arguably the first person to bring this new technological …

The bacterial cell wall is essential for viability, but despite its ability to withstand internal
turgor must remain dynamic to permit growth and division. Peptidoglycan is the major cell …

As proof-of-principle for generating superresolution structural information from DNA we
applied a method of localization microscopy utilizing photoblinking comparing intercalating …

We present a single-molecule tool called the CoPro (concentration of proteins) method that
uses millisecond imaging with convolution analysis, automated image segmentation and …

The resistance of DNA to stretch, twist and bend is broadly well estimated by experiments
and is important for gene regulation and chromosome packing. However, their sequence …

The double-helical structure of DNA results from canonical base pairing and stacking
interactions. However, variations from steady-state conformations resulting from mechanical …

Single-molecule narrow-field microscopy is a versatile tool to investigate a diverse range of
protein dynamics in live cells and has been extensively used in bacteria. Here, we describe …

We present a novel experimental setup in which magnetic and optical tweezers are
combined for torque and force transduction onto single filamentous molecules in a …