Single-molecule localization microscopy
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …
techniques that dramatically improve spatial resolution over standard, diffraction-limited …
Cryo-electron tomography on focused ion beam lamellae transforms structural cell biology
Cryogenic electron microscopy and data processing enable the determination of structures
of isolated macromolecules to near-atomic resolution. However, these data do not provide …
of isolated macromolecules to near-atomic resolution. However, these data do not provide …
Whole-cell organelle segmentation in volume electron microscopy
Cells contain hundreds of organelles and macromolecular assemblies. Obtaining a
complete understanding of their intricate organization requires the nanometre-level, three …
complete understanding of their intricate organization requires the nanometre-level, three …
Super-resolution microscopy for structural cell biology
Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …
The promise and the challenges of cryo‐electron tomography
M Turk, W Baumeister - FEBS letters, 2020 - Wiley Online Library
Structural biologists have traditionally approached cellular complexity in a reductionist
manner in which the cellular molecular components are fractionated and purified before …
manner in which the cellular molecular components are fractionated and purified before …
[HTML][HTML] ER-to-Golgi protein delivery through an interwoven, tubular network extending from ER
Cellular versatility depends on accurate trafficking of diverse proteins to their organellar
destinations. For the secretory pathway (followed by approximately 30% of all proteins), the …
destinations. For the secretory pathway (followed by approximately 30% of all proteins), the …
Bringing structure to cell biology with cryo-electron tomography
Recent advances in cryo-electron microscopy have marked only the beginning of the
potential of this technique. To bring structure into cell biology, the modality of cryo-electron …
potential of this technique. To bring structure into cell biology, the modality of cryo-electron …
Volume electron microscopy
Life exists in three dimensions, but until the turn of the century most electron microscopy
methods provided only 2D image data. Recently, electron microscopy techniques capable of …
methods provided only 2D image data. Recently, electron microscopy techniques capable of …
ESCRT-mediated membrane repair protects tumor-derived cells against T cell attack
Cytotoxic T lymphocytes (CTLs) and natural killer cells kill virus-infected and tumor cells
through the polarized release of perforin and granzymes. Perforin is a pore-forming toxin …
through the polarized release of perforin and granzymes. Perforin is a pore-forming toxin …
The transcription factor activity gradient (TAG) model: contemplating a contact-independent mechanism for enhancer–promoter communication
How distal cis-regulatory elements (eg, enhancers) communicate with promoters remains an
unresolved question of fundamental importance. Although transcription factors and cofactors …
unresolved question of fundamental importance. Although transcription factors and cofactors …