What is a transcriptional burst?
E Tunnacliffe, JR Chubb - Trends in Genetics, 2020 - cell.com
The idea that gene activity can be discontinuous will not surprise many biologists–many
genes are restricted in when and where they can be expressed. Yet during the past 15 …
genes are restricted in when and where they can be expressed. Yet during the past 15 …
What's luck got to do with it: single cells, multiple fates, and biological nondeterminism
The field of single-cell biology has morphed from a philosophical digression at its inception,
to a playground for quantitative biologists, to a major area of biomedical research. The last …
to a playground for quantitative biologists, to a major area of biomedical research. The last …
Imaging mRNA in vivo, from birth to death
RNA is the fundamental information transfer system in the cell. The ability to follow single
messenger RNAs (mRNAs) from transcription to degradation with fluorescent probes gives …
messenger RNAs (mRNAs) from transcription to degradation with fluorescent probes gives …
Single-molecule kinetics in living cells
J Elf, I Barkefors - Annual review of biochemistry, 2019 - annualreviews.org
In the past decades, advances in microscopy have made it possible to study the dynamics of
individual biomolecules in vitro and resolve intramolecular kinetics that would otherwise be …
individual biomolecules in vitro and resolve intramolecular kinetics that would otherwise be …
SCALE: modeling allele-specific gene expression by single-cell RNA sequencing
Allele-specific expression is traditionally studied by bulk RNA sequencing, which measures
average expression across cells. Single-cell RNA sequencing allows the comparison of …
average expression across cells. Single-cell RNA sequencing allows the comparison of …
Stochastic kinetics of nascent RNA
The stochastic kinetics of transcription is typically inferred from the distribution of RNA
numbers in individual cells. However, cellular RNA reflects additional processes …
numbers in individual cells. However, cellular RNA reflects additional processes …
Distribution shapes govern the discovery of predictive models for gene regulation
Despite substantial experimental and computational efforts, mechanistic modeling remains
more predictive in engineering than in systems biology. The reason for this discrepancy is …
more predictive in engineering than in systems biology. The reason for this discrepancy is …
Extrinsic noise and heavy-tailed laws in gene expression
Noise in gene expression is one of the hallmarks of life at the molecular scale. Here we
derive analytical solutions to a set of models describing the molecular mechanisms …
derive analytical solutions to a set of models describing the molecular mechanisms …
Coupling gene expression dynamics to cell size dynamics and cell cycle events: Exact and approximate solutions of the extended telegraph model
The standard model describing the fluctuations of mRNA numbers in single cells is the
telegraph model which includes synthesis and degradation of mRNA, and switching of the …
telegraph model which includes synthesis and degradation of mRNA, and switching of the …
Frequency domain analysis of fluctuations of mRNA and protein copy numbers within a cell lineage: theory and experimental validation
The stochasticity of gene expression is manifested in the fluctuations of messenger
ribonucleic acid and protein copy numbers within a cell lineage over time. While data of this …
ribonucleic acid and protein copy numbers within a cell lineage over time. While data of this …