Protoplast fusion technology has been utilized in many crops to generate allotetraploid
somatic hybrids, and sometimes autotetraploids as a byproduct of the process. A brief history …

Developing CRISPR/Cas9-mediated non-transgenic mutants in asexually propagated
perennial crop plants is challenging but highly desirable. Here, we report a highly useful …

Abstract The avocado, Persea americana Mill., is one of the most traded tropical fruits in the
international market, furthermore, demand has significantly grown over the last decade …

Transient gene expression in plant protoplasts is a powerful tool for analyzing gene function
and for performing biotechnical manipulations. Here we report the isolation of viable …

Oil palm protoplasts are suitable as a starting material for the production of oil palm plants
with new traits using approaches such as somatic hybridization, but attempts to regenerate …

High yields of protoplasts were isolated from litchi embryogenic suspensions, which were
maintained by alternative culture in liquid and on solid media containing silver thiosulfate …

Plant somatic hybridization via protoplast fusion has become an important tool in plant
improvement, allowing researchers to combine somatic cells (whole or partial) from different …

Embryogenic avocado cultures derived from 'Hass' protoplasts were genetically transformed
with the plant defensin gene (pdf1. 2) driven by the CaMV 35S promoter in pGPTV with uidA …

This report describes a protocol for the regeneration of plants from protoplasts isolated from
proembryogenic masses (PEMs) in a suspension culture derived from the nucellar callus of …

Apple belongs to the genus Malus within the Rosaceae family, which includes some of the
most commercially prominent fruit crops: pears, plums, peaches, nectarines, prunes …