Detection of foodborne pathogens by qPCR: a practical approach for food industry applications

MJ Chapela, A Garrido-Maestu… - Cogent Food & …, 2015 - Taylor & Francis
Microbiological analysis of food is an integrated part of microbial safety management in the
food chain. Monitoring and controlling foodborne pathogens are traditionally carried out by …

A new multiplex real-time PCR developed method for Salmonella spp. and Listeria monocytogenes detection in food and environmental samples

A Garrido, MJ Chapela, B Román, P Fajardo, J Lago… - Food control, 2013 - Elsevier
Despite efforts done by industries some well known foodborne pathogens, like Salmonella
spp. and Listeria monocytogenes, continue to be a challenge to public health institutions …

Development of a 3-plex droplet digital PCR for identification and absolute quantification of Salmonella and its two important serovars in various food samples

Z Fang, X Zhou, X Wang, X Shi - Food Control, 2023 - Elsevier
Salmonella is one of the most common foodborne pathogens that cause diarrhea in human,
in which Enteritidis and Typhimurium are the top serovars frequently isolated from foodstuffs …

In-house validation of a multiplex real-time PCR method for simultaneous detection of Salmonella spp., Escherichia coli O157 and Listeria monocytogenes

A Garrido, MJ Chapela, B Román, P Fajardo… - International Journal of …, 2013 - Elsevier
A wide variety of qPCR methods currently exist for Salmonella spp., Escherichia coli O157
and Listeria monocytogenes detection. These methods target several genes and use …

Simultaneous detection of Listeria monocytogenes and Salmonella spp. in dairy products using real time PCR-melt curve analysis

J Singh, VK Batish, S Grover - Journal of food science and technology, 2012 - Springer
The present investigation reports development of post real time PCR (RTi-PCR)-melt curve
analysis for simultaneous detection of Listeria monocytogenes and Salmonella spp. The …

Validation of Real-Time PCR and Enzyme-Linked Fluorescent Assay-Based Methods for Detection of Salmonella spp. in Chicken Feces Samples

D Tomas, A Rodrigo, M Hernandez, MA Ferrus - Food Analytical Methods, 2009 - Springer
A comparative study of enzyme-linked fluorescent assay (ELFA)-based methods and real-
time polymerase chain reaction (PCR)-based methods using three and two different sample …

Development of a multiplex real-time PCR method for pathogenic Vibrio parahaemolyticus detection (tdh+ and trh+)

A Garrido, MJ Chapela, M Ferreira, M Atanassova… - Food Control, 2012 - Elsevier
A multiplex real-time PCR (RT-PCR) method was developed in this work for pathogenic
Vibrio parahaemolyticus detection in water and food samples. This bacterium is found in the …

Multiplex Real-time PCR for the Simultaneous Detection of Salmonella spp. and Listeria monocytogenes in Food Samples

O Ruiz-Rueda, M Soler, L Calvó, JL García-Gil - Food analytical methods, 2011 - Springer
In this study, we have developed a rapid method for the simultaneous detection of Listeria
monocytogenes and Salmonella spp. in foods, combining culture enrichment and a …

In-house validation of novel multiplex real-time PCR gene combination for the simultaneous detection of the main human pathogenic vibrios (Vibrio cholerae, Vibrio …

A Garrido-Maestu, MJ Chapela, E Peñaranda… - Food Control, 2014 - Elsevier
In recent years the incidence of vibriosis has greatly increased, raising the concern among
consumers about the innocuity of certain food products. Previous studies demonstrated …

Direct detection of Salmonella without pre-enrichment in milk, ice-cream and fruit juice by PCR against hilA gene

SA Marathe, R Chowdhury, R Bhattacharya… - Food Control, 2012 - Elsevier
A novel PCR based assay was devised to specifically detect contamination of any
Salmonella serovar in milk, fruit juice and ice-cream without pre-enrichment. This method …