The rapid increase in the number of diabetic patients globally and exploration of alternate
insulin delivery methods such as inhalation or oral route that rely on higher doses, is bound …

Yeast expression systems have been successfully used for over 20 years for the production
of recombinant proteins. With the growing interest in recombinant protein expression for …

The methylotrophic yeast Pichia pastoris (syn. Komagataella spp.) is one of the most
important production systems for heterologous proteins. After the first genome sequences …

Over the past 100 years, industrial biotechnology has grown into a multibillion dollar market,
which now has even begun to include large parts of chemical, material, and fuel production …

The alcohol oxidase 1 promoter (P AOX1) of Pichia pastoris is commonly used for high level
expression of recombinant proteins. While the safety risk of methanol and tough process …

Pichia pastoris is an efficient host for the expression and secretion of heterologous proteins
and the most important feature of P. pastoris is the existence of a strong and tightly regulated …

The success of Pichia pastoris as a heterologous expression system lies predominantly in
the impressive yields that can be achieved due to high volumetric productivity. However, low …

Aims: This study attempted to fully explore the expression potentials of Pichia pastoris for
producing porcine insulin precursor (PIP) through PIP copy number optimization. Methods …

Background The prevalence of diabetes is predicted to rise significantly in the coming
decades. A recent analysis projects that by the year 2030 there will be~ 366 million diabetics …

As an alternative carotenoid producer, non-carotenogenic Pichia pastoris was chosen for a
reddish carotenoid lycopene production because it can grow to high cell density without …