Engineering the metabolic landscape of microorganisms for lignocellulosic conversion

JM Peña-Castro, KM Muñoz-Páez… - Microorganisms, 2023 - mdpi.com
Bacteria and yeast are being intensively used to produce biofuels and high-added-value
products by using plant biomass derivatives as substrates. The number of microorganisms …

Engineering a new-to-nature cascade for phosphate-dependent formate to formaldehyde conversion in vitro and in vivo

M Nattermann, S Wenk, P Pfister, H He, SH Lee… - Nature …, 2023 - nature.com
Formate can be envisioned at the core of a carbon-neutral bioeconomy, where it is produced
from CO2 by (electro-) chemical means and converted into value-added products by …

Creating new-to-nature carbon fixation: A guide

H Schulz-Mirbach, B Dronsella, H He, TJ Erb - Metabolic Engineering, 2023 - Elsevier
Synthetic biology aims at designing new biological functions from first principles. These new
designs allow to expand the natural solution space and overcome the limitations of naturally …

Identification of 2-Hydroxyacyl-CoA synthases with high acyloin condensation activity for orthogonal one-carbon bioconversion

SH Lee, A Chou, M Nattermann, F Zhu… - ACS …, 2023 - ACS Publications
One-carbon (C1) compounds are emerging as cost-effective and potentially carbon-negative
feedstocks for biomanufacturing, which require efficient, versatile metabolic platforms for the …

Discovery and remodeling of Vibrio natriegens as a microbial platform for efficient formic acid biorefinery

J Tian, W Deng, Z Zhang, J Xu, G Yang, G Zhao… - Nature …, 2023 - nature.com
Formic acid (FA) has emerged as a promising one-carbon feedstock for biorefinery.
However, developing efficient microbial hosts for economically competitive FA utilization …

Metal-free organocatalytic S-formylation of thiols using CO2

S Maji, A Das, MM Bhatt, SK Mandal - Nature Catalysis, 2024 - nature.com
S-formylation of thiols is achieved via an enzymatic process catalysed by formate
dehydrogenase enzyme that fixes CO2 onto substrates, which plays a crucial role in …

Engineering the reductive glycine pathway: A promising synthetic metabolism approach for c1-assimilation

NJ Claassens, A Satanowski, VR Bysani… - One-Carbon Feedstocks …, 2022 - Springer
In recent years the reductive glycine pathway (rGlyP) has emerged as a promising pathway
for the assimilation of formate and other sustainable C1-feedstocks for future biotechnology …

Formaldehyde: An Essential Intermediate for C1 Metabolism and Bioconversion

M Jia, M Liu, J Li, W Jiang, F Xin, W Zhang… - ACS Synthetic …, 2024 - ACS Publications
Formaldehyde is an intermediate metabolite of methylotrophic microorganisms that can be
obtained from formate and methanol through oxidation–reduction reactions. Formaldehyde …

Design, construction and optimization of formaldehyde growth biosensors with broad application in biotechnology

K Schann, J Bakker, M Boinot, P Kuschel… - Microbial …, 2024 - Wiley Online Library
Formaldehyde is a key metabolite in natural and synthetic one‐carbon metabolism. To
facilitate the engineering of formaldehyde‐producing enzymes, the development of …

[HTML][HTML] A synthetic cell-free pathway for biocatalytic upgrading of one-carbon substrates

GM Landwehr, B Vogeli, C Tian, B Singal, A Gupta… - …, 2024 - pmc.ncbi.nlm.nih.gov
Biotechnological processes hold tremendous potential for the efficient and sustainable
conversion of one-carbon (C1) substrates into complex multi-carbon products. However, the …